Full Description
Immuno-oncology and immunotherapy, Part F, Volume 201 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including an Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation, Cancer cyro-immunotherapy: Models and methods, Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology, In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes, and much more.
Additional sections cover the Analysis of the tumor microenvironment using imaging mass cytometry data, Saphenous vein blood collection for different immune analyses of living mice, Use of mouse KO models to validate the specificity of monoclonal antibodies, Imaging mass cytometry to analyze the immune TME, Autoimmune arthritis: Transgenic mouse models and methods, Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments, and more.
Contents
1. Generation and functional evaluation of STAb-T cells
Luis Alvarez-Vallina
2. A syngeneic orthotopic mouse model of metastatic colorectal cancer
Christel Devaud
3. High-parameter T-cell spectral phospho flow cytometry
Joshua Brody and Gvantsa Pantsulaia
4. Measuring T-cell/CAR T-cell bystander tumor killing in vitro and in vivo
Joshua Brody, Matthew Lin and Daniel Charytonowicz
5. Quantification and functional assessment of antigen(-) cancer cell variants
Joshua Brody, Gvantsa Pantsulaia, Ivan Odak and Xie Xinping
6. T-cell and CAR T-cell Cas9-RNP/nucleofection-mediated gene editing
Joshua Brody, Gvantsa Pantsulaia and Moah Sohn
7. Peripheral blood mononuclear cell (PBMC)- based functional evaluation of human T cell response to suppressive cells and immune-oncology therapeutics
Maria G. Castro, Kaushik Banerjee, Pedro R. Lowenstein, Mahmoud Alghamri, Brandon McClellan and Jorge Peña Agudelo
8. Integrative analysis of lung multiparametric flow cytometry data to study immune cell dynamics in tumors
Romina Goldszmid and Romina Araya
9. Multiparametric staining: Combined application of immunofluorescence and in-situ hybridization
Barbara Seliger, Marcus Bauer, Andreas Wilfer, Clara Zöllig and Claudia Wickenhauser
10. Extracellular matrix and morphology assessment method on live 3D spheroids of thyroid carcinoma
Barbara Seliger, Mario Udinotti, Udo Sieboldts and Christoforos Vaxevanis
11. Hema-CRISPR: A clone-based protocol for effective genetic editing of hematopoietic stem cells
Barbara Seliger, Christoforos Vaxevanis, Mario Udinotti, Dimitris Kokoretsis, Andreas Wilfer, Marcus Bauer and Claudia Wickenhauser
12. Patient-derived models of tumor-immune cell interactions
Zlatko Trajanoski
13. MDSC isolation from human tumors
Viktor Umansky, Feyza Gul Ozbay Kurt and Jochen Utikal
14. Extracellular vesicles isolation from human maternal fluids
Sheila Spada
15. Finding a Needle in a Haystack: Measures of Circulating Tumor DNA (ctDNA) to Guide Treatment Decisions in Oncology
Lotze, Michael T, Genia Dubrovsky, Jeff Szymanski, Aadel A. Chaudhuri, Jose Zevallos, Noah Earland, Peter Harris and Pradeep Chauhan
