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Full Description
Immuno-oncology and immunotherapy, Part E, Volume 199 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including an Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation, Cancer cyro-immunotherapy: Models and methods, Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology, In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes, and much more.
Additional sections cover the Analysis of the tumor microenvironment using imaging mass cytometry data, Saphenous vein blood collection for different immune analyses of living mice, Use of mouse KO models to validate the specificity of monoclonal antibodies, Imaging mass cytometry to analyze the immune TME, Autoimmune arthritis: Transgenic mouse models and methods, Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments, and more.
Contents
1. Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation
Douglas McNeel
2. Cancer cyro-immunotherapy: Models and methods
George Prendergast
3. Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology
Ileana Mauldin and Leon Zheng
4. In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes
Mario Tarantini, Anna Lisa GIULIANI, Valentina Vultaggio-Poma, Simonetta Falzoni, Elena Adinolfi and Francesco Di Virgilio
5. Analysis of the tumor microenvironment using imaging mass cytometry data
Zlatko Trajanoski and Zuzana Loncova
6. Saphenous vein blood collection for different immune analyses of living mice
Ana Carolina Martinez Torres, Kenny Misael Calvillo-Rodriguez, Reyes Tamez-Guerra, Cristina Rodriguez-Padilla and Kassandra Ofelia Rodriguez-Aguillon
7. Use of mouse KO models to validate the specificity of monoclonal antibodies
George Prendergast, Lisa D. Laury-Kleintop, Summer Sedano and Kaylend Manley
8. Imaging mass cytometry to analyze the immune TME
Louis M. Weiner
9. Autoimmune arthritis: Transgenic mouse models and methods
Laura Mandik-Nayak, Lauren Merlo and Weidan Peng
10. Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments
Maha Ayyoub, Clara Maria SCARLATA, Virginie Feliu and Christel Devaud
11. Generation and functional evaluation of bispecific antibodies
Luis Alvarez-Vallina, Antonio Tapia Galisteo, Anaïs Jiménez-Reinoso, Rodrigo Lázaro Gorines and Belén Blanco
12. Isolation and phenotype analysis of intraepithelial lymphocytes from the mouse colon
Laura Bracci, Alessia Fiore, Michela Tozzi, Paola Rinzo, Daniele Macchia, Massimo Spada and Alessia Fabbri
13. Analysis of single cell TCR repertoires and gene expression from multi-modal scRNA-seq data
Dietmar Rieder, Christina Plattner and Gregor Sturm
14. Analysis of the tumor immune microenvironment of brain tumors by flow-cytometry
Claire I. Vanpouille-Box
15. Assessment of in vitro interactions between eosinophils and cancer cells by imaging-enhanced flow cytometry and time-lapse microscopy.
Fabrizio Mattei, Giovanna Schiavoni, Caterina Antonucci, Adriana Rosa Gambardella and Valentina Tirelli
16. Pharmacological screening to identify potential regulators of antigen presentation by dendritic cells
Peng LIU, Liwei Zhao, Guido Kroemer, Oliver Kepp, Yuhong Pan and Shuai Zhang
