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Full Description
Immuno-oncology and immunotherapy, Part G, Volume 202 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of timely topics, including an Evaluation of agents that affect anti-tumor function of CD8+ T cells at activation, Cancer cyro-immunotherapy: Models and methods, Methods for analysis of cancer- and TLS-associated immune infiltrates by multiplex immunofluorescence histology, In-vivo measurement of the extracellular ATP concentration by bio-luminescent probes, and much more.
Additional sections cover the Analysis of the tumor microenvironment using imaging mass cytometry data, Saphenous vein blood collection for different immune analyses of living mice, Use of mouse KO models to validate the specificity of monoclonal antibodies, Imaging mass cytometry to analyze the immune TME, Autoimmune arthritis: Transgenic mouse models and methods, Assessment of human and mouse tumor-antigen specific CD8+ T cells by multimer staining in multiple compartments, and more.
Contents
1. In situ imaging of pulmonary invariant Natural Killer T cells in precision cut lung slices
Claudia Burrello, Karin E. de Visser and Tom Langelaar
2. Immunofluorescent microscopy to quantify and characterize chromosomal instability
Pippa F. Cosper, Kathryn Jones, Yeseo Choi, Radchanon Leelasukseree, Nikki Messick, Ava Bryan and Sophie Bice
3. Spatial techniques for understanding the tumour microenvironment
Florent Ginhoux, Margaux GARDET and Kevin Mulder
4. Very high-flex immunophenotyping using co-linear cytofluorometric evaluation
Benjamin Izar
5. Large-scale CRISPR-Cas9 screens to define regulators of immune checkpoint biology
Benjamin Izar
6. Assessment of cytoplasmic dsDNA for cellular immunogenicty
Rongze Olivia Lu, Raymond Sun, Oishika Das and Isha Mondol
7. Harmonization of a multiparametric flow cytometry panel for immunological monitoring on PBMC and whole blood samples: a real-life example
Iole Macchia Sr., Francesca Urbani and Valentina La Sorsa
8. Quantitative assessment of arterial remodeling from confocal microscopy image analysis of the mid-gestation mouse decidua
Alexander J. Muller and Dema Alghabban
9. Phenotypic characterization and function of tumor infiltrating and peripheral B cells in cancer patients
Paola Nistico, Maria Rosado, Giuseppe Frisullo, Giulia Campo and Miriam Petrella
10. Methodological Advances to analyze Cancer-Associated Fibroblast-derived ECM effects on Macrophage Polarization
Paola Nistico, Annalisa Tocci, Nicla Porciello, Belinda Palermo, Lorenzo Valenti, Anna Di Carlo and Francesca Di Modugno
11. Lymphocyte migration/invasion assays
Louis M. Weiner, Rachael Maynard and Alex Lekan
12. Flow-cytometry-based assay to study CD8+ T cell cytotoxicity against tumor cells.
Roberta Zappasodi, Alexey V Sarapulov and Marc-Anthony Rodriguez
13. High dimensional flow cytometry for conventional and unconventional T cell populations
Maria Paula Roberti, Andrea Leufgen, Conrad Rauber, Annika Steitz, Paula Daza and Maria Paz Gavilan
14. Optimized Phospho-Flow Cytometry for Quantitative Analysis of Early TCR-Proximal Signaling: Implications for T Cell Function in Onco-Immunology
Paola Nistico, Nicla Porciello, Annalisa Tocci, Giulia Campo and Belinda Palermo
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