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Full Description
Cellular Senescence and Aging, Volume 181 in the Methods in Cell Biology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegans, Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholino, Methods for detection of mitochondrial reactive oxygen species in senescent cells, Assessment of cell cycle progression and mitotic slippage by videomicroscopy, The original colorimetric method to detect cellular senescence, and more.
Additional sections cover Assessing microbiota composition in the context of aging, Assessing chronological aging in Saccharomyces cerevisiae, Image processing and supervised machine learning for retinal microglia characterization in senescence, Measuring telomerase activity using TRAP assays, High throughput assessment of cellular senescence, Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events, Dynamic and scalable assessment of the senescence-associated secretory phenotype (SASP), Flow cytometry-assisted quantification of cell cycle arrest in cancer cells treated with CDK4/6 inhibitors, and more.
Contents
1. Assessing polyglutamine tract aggregation in the nematode Caenorhabditis elegans
Aggeliki Sotiriou, Christina Ploumi, Nikolaos Charmpilas and Nektarios Tavernarakis
2. Generation of glial cell-derived neurotrophic factor (gdnf) morphants in zebrafish larvae by cerebroventricular microinjection of vivo morpholino
Suzita Mohd Noor, Chee Ern David Wong, Pooi-Fong Wong and Anwar Norazit
3. Methods for detection of mitochondrial reactive oxygen species in senescent cells
Fenniche Salma, Oubaddou Yassire, Bakri Youssef, Dupuy Corinne and Ameziane El Hassani Rabii
4. Assessment of cell cycle progression and mitotic slippage by videomicroscopy
Luca Mattiello, Sara Soliman Abdel Rehim, Gwenola Manic and Ilio Vitale
5. The original colorimetric method to detect cellular senescence
Manjari Dimri and Goberdhan P. Dimri
6. Assessing microbiota composition in the context of aging
Diana Campos-Iglesias, José M.P. Freije and Carlos López-Otín
7. Assessing chronological aging in Saccharomyces cerevisiae
Adina Schulze, Andreas Zimmermann, Katharina Kainz, Nadine B. Egger, Maria A. Bauer, Frank Madeo and Didac Carmona-Gutierrez
8. Image processing and supervised machine learning for retinal microglia characterization in senescence
Soyoung Choi, Daniel Hill, Jonathan Young and Maria Francesca Cordeiro
9. Measuring telomerase activity using TRAP assays
Gabriele Saretzki
10. High throughput assessment of cellular senescence
Giulia Cerrato, Allan Sauvat, Felix Peyre, Oliver Kepp and Guido Kroemer
11. Detection of radiation-induced senescence by the Debacq-Chainiaux protocol: Improvements and upgrade in the detection of positive events
V. Paget, O. Guipaud, A. François and F. Milliat
12. Dynamic and scalable assessment of the senescence-associated secretory phenotype (SASP)
Nicolas Malaquin and Francis Rodier
13. Flow cytometry-assisted quantification of cell cycle arrest in cancer cells treated with CDK4/6 inhibitors
Vanessa Klapp, Norma Bloy, Carlos Jiménez-Cortegana, Aitziber Buqué and Giulia Petroni
14. Mechanistic exploration of autophagy and aging by RNA interference
Hui Pan, Oliver Kepp and Guido Kroemer
