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Full Description
When the first edition of this book was published in 1950, it predated the publication of the double-helical structure of DNA by three years. It is not, therefore, surprizing that nothing of the original book remains in the current edition. Indeed, such is the pace of change in the field of nucleic acids that less than 50% of material incorporated into the 1986 edition has been retained. The book aims at the advanced undergraduate and at graduates that are undertaking course work or requiring an in-depth background for their research. It also aims to provide the established scientist with a single text that permits updating across the whole field from DNA structure, replication and repair, through gene expression and its control to protein synthesis. Every chapter is accompanied by thorough referencing that enables the reader to evaluate personally the data and methodology that cannot be included in the text. In an attempt to keep this list within bounds, references are limited to about ten per page and, to accommodate the more recent literature, many of the older references have been left out in this latest edition.
Contents
1 Introduction.- 2 The structure of the nucleic acids.- 3 Genomes of eukaryotes, bacteria and viruses: chromosome organization.- 4 Degradation and modification of nucleic acids.- 5 The metabolism of nucleotides.- 6 Replication of DNA.- 7 Repair, recombination and rearrangement of DNA.- 8 The arrangement of genes.- 9 RNA biosynthesis.- 10 Control of transcription.- 11 Processing of RNA transcripts and its control.- 12 The translation of mRNA: protein synthesis.- Appendix: methods of studying nucleic acids.- A.1 Occurrence and chemical analysis.- A.1.1 Chemical determination of nucleic acids in tissues.- A.1.2 Analysis of base composition and nearest-neighbour frequency.- A.1.3 Estimation of the molecular weight of DNA.- A.2 Isolation and separation of nucleic acids.- A.2.1 Isolation and separation of DNA.- A.2.2 Isolation and separation of RNA.- A.3 Hybridization of nucleic acids.- A.4 Methods of labelling nucleic acids.- A.4.1 General labelling methods.- A.4.2 End-labelling methods.- A.5 Determination of nucleic acid sequences.- A.5.1 Determination of DNA sequences.- A.5.2 Determination of RNA sequences.- A.6 Restriction mapping of DNA.- A.7 The polymerase chain reaction.- A.8 Cloning DNA.- A.8.1 The principles.- A.8.2 The major cloning vectors.- A.8.3 cDNA cloning.- A.8.4 Genomic cloning.- A.9 Analysis and manipulation of cloned DNA.- A.9.1 Mapping of RNA transcripts.- A.9.2 Identification of regions of DNA that interact with proteins.- A.9.3 Expression.- A.10 Chemical synthesis of oligonucleotides.- A.11 Cell-free systems for transcription and translation.- A.12 The use of inhibitors in the study of gene expression.- References.
