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Full Description
Preparative Layer Chromatography explains how this method is used for separating large quantities of mixtures containing a wide variety of important compounds. It offers a broad review of preparative layer chromatography (PLC) applications and adaptable working procedures for microseparations involving organic, inorganic, and organometallic compounds.
The book contains theoretical background, chemical principles, and relevance of preparative layer chromatography (PLC) to a wide range of applications, particularly in the study of pharmaceuticals and biochemistry. Written by many of the best known and most knowledgeable specialists in the field, the chapters describe all the necessary techniques, current procedures, and superior strategies for selecting the most suitable eluents and designing application-specific PLC systems based on the data being sought. They provide comprehensive instructions, surrounding issues, and suggestions for optimizing optional working techniques within the framework of PLC. The book also provides a complete coverage of bulk sorbents and precoated chromatographic plates available on the international market.
A comprehensive, yet accessible source of information, Preparative Layer Chromatography is a relevant and practical text for experienced as well as novice researchers and practitioners involved in analytical, environmental, geochemical, biological, medicinal, and pharmaceutical analysis.
Contents
Introduction. Adsorption Planar Chromatography in the Non-linear Range: Selected Drawbacks and Selected Guidelines. Sorbents and Precoated Layers in PLC. Selection and Optimization of the Mobile Phase for PLC. Sample Application and Development of Layers. On Methodical Possibilities of the Horizontal Chambers in PLC. Location of Separated Zones by Use of Visualization Reagents, UV Absorbance on Layers Containing a Fluorescent Indicator, and Densitometry. Additional Detection Methods for Separated Zones and Recovery of Substances from the Layer. Medical Applications of PLC. PLC of Hydrophobic Vitamins. PLC of Natural Mixtures. Application of PLC to Lipids. The Use of PLC for Separation of Natural Pigments. Application of PLC to Inorganics and Organometallics. PLC in a Clean-up and Group Fractionaction of Geochemical Samples: A Review of the Commonly Applied Techniques. The Use of PLC for Isolation and Identification of Unknown Compounds from the Frankincense Resin: Strategies for Finding Marker Substances.
