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Full Description
Next Generation Sequencing (NGS) Technology in DNA Analysis explains and summarizes next generation sequencing (NGS) technological applications in the field of forensic DNA analysis. The book covers the transition from capillary electrophoresis (CE)-based technique to NGS platforms and the fundamentals of NGS technologies, applications, and advances. Sections provide an overview of NGS technology and forensic science, including information on processing biological samples for forensic analysis, sequence analysis, and data analysis software as well as the analysis of NGS data. The book explores the valuable applications of NGS-based forensic DNA analysis and covers the validations and interpretation guidelines of NGS workflows.
With chapter contributions from an international array of experts and the inclusion of practical case studies, this book is a useful reference for academicians and researchers in genetics, biotechnology, bioinformatics, biology, and medicine as well as forensic DNA scientists and practitioners who aim to learn, use, apply, and validate NGS-based technologies.
Contents
SECTION 1 Useful applications of NGS-based forensic DNA analysis
1. Transition of capillary electrophoresis to next generation sequencing for forensic DNA analysis: Need of the hour
2. Using conventional STR technology in analyzing biological transfer evidence
3. Overview of NGS platforms and technological advancements for forensic applications
4. Processing of biological samples for forensic NGS analysis
5. Commercial kits commonly used for NGS based forensic DNA analysis
6. Applications of nanopore sequencing for forensic analysis
7. Microhaplotypes analysis for human identification using next-generation sequencing (NGS)
8. Tools and techniques of using NGS technology in population studies
SECTION 2 Useful applications of NGS-based forensic DNA analysis
9. Applications of NGS in analysis of autosomal STRs
10. Differentiating monozygotic twins using NGS
11. Application of NGS in maternal genome analysis in ancient human remains
12. Application of NGS technology for parentage testing and relatedness analysis
13. Forensic relevance of SNP analysis in next-generation sequencing
14. Forensic analysis of hair using NGS technology
15. Forensic DNA phenotyping using next-generation sequencing
16. Forensic DNA phenotyping in the next-generation sequencing era
17. Forensic applications of epigenetic (DNA methylation) markers through NGS
18. Forensic applications of NGS-based microRNA analysis
19. NGS profiling of water and soil microbial DNA in forensic science
20. Applications of NGS in analysis of challenging samples
21. Detection of human body fluid through mRNA analysis using NGS
22. NGS-based detection and differentiation of forensically relevant body fluids using conventional, molecular, and microbial techniques
23. Application of next-generation sequencing technology and investigative genetic genealogy to cold cases
SECTION 3 Challenges in implementing NGS in forensic laboratories
24. Troubleshooting and challenges of Next-generation sequencing technology in forensic use
25. Validation of NGS for casework at forensic DNA laboratories
26. Challenges in using genetic genealogy in forensics
27. The role of artificial intelligence and machine learning in NGS
28. Ethical issues of forensic application of NGS technology
29. Ethical, legal, and social challenges of next-generation sequencing technologies (NGS) in forensic criminal identification
