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Full Description
Correlative Light and Electron Microscopy V, Volume 187 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Orthotopic brain tumor models derived from glioblastoma stem-like cells, RNA sequencing in hematopoietic stem cells, Generation of inducible pluripotent stem cells from human dermal fibroblasts, In vitro preparation of dental pulp stem cell grafts combined with biocompatible scaffolds for tissue engineering, Gene expression knockdown in chronic myeloid leukemia stem cells, Identification and isolation of slow-cycling GSCs, Assessment of CD133, EpCAM, and much more.
Contents
1. How to Apply the Broad Toolbox of Correlative Light and Electron Microscopy to Address a Specific Biological Question
Thomas Müller-Reichert, Erin M. Tranfield, Gunar Fabig and Thomas Kurth
2. Some tips and tricks for a correlative light electron microscopy workflow using stable expression of fluorescent proteins
Paul Verkade
3. Targeting membrane receptors with fluoronanogold probes for high resolution correlative microscopy.
Monica Fernandez Monreal
4. Correlative light and electron microscopy at defined cell cycle stages in a controlled environment
Shotaro Otsuka
5. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) culture and sample preparation for correlative light electron microscopy
Paul Verkade, Maximillian Erdmann, Isobel Web, Lorna Hodgson and Andrew Davidson
6. Tissue CLEM
Christine Longin
7. Array tomography of in vivo labeled synaptic receptors
Christian Stigloher and Sebastian Britz
8. Correlative cryo-microscopy pipelines for in situ cellular studies
Anna Sartori and Chiara Zurzolo
9. Building a super-resolution fluorescence cryomicroscope
Thom Sharp
10. Analysis of super resolution CLEM data
Thom Sharp
11. Nanometer targeting using cryo super resolution
Laura Carolina Zanetti-Domingues
12. Laboratory Based Correlative Cryo-Soft X-Ray Tomography and Cryo-Fluorescence Microscopy
Kenneth Fahy
