合成DNA輸送系<br>Synthetic DNA Delivery Systems (Biotechnology Intelligence Unit, V. 7)

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合成DNA輸送系
Synthetic DNA Delivery Systems (Biotechnology Intelligence Unit, V. 7)

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  • 製本 Hardcover:ハードカバー版/ページ数 150 p.
  • 言語 ENG
  • 商品コード 9780306477010
  • DDC分類 660.65

基本説明

A comprehensive resource on DNA transfection. The use of histidine-rich peptides and polypeptides as DNA delivery systems and self-assembled delivery systems based on cationic lipids and polymers are discussed.

Full Description

DNA delivery into cells is a rapidly developing area in gene therapy and biotechnology. Moreover, it is a powerful research tool to determine gene structure, regulation, and function.

Viral methods of DNA delivery are well-characterized and efficient, but little is known about the toxicity and immunogenecity of viral vectors. As a result, non-viral, transfection methods of DNA delivery are of increasing interest.

Synthetic DNA Delivery Systems is a comprehensive and current resource on DNA transfection. The use of histidine-rich peptides and polypeptides as DNA delivery systems and self-assembled delivery systems based on cationic lipids and polymers are discussed. Targeted delivery to organelles, tumor cells and dendritic cells comprise an important topic.

Contents

I: Introduction; D. Luo, W.M. Saltzman. II: Overcoming Cellular Barriers along the DNA Delivery Pathway. Outside of the cell: Supramolar assemblies of DNA delivery systems; Pitard. At the cell surface: Engineering the surface properties of synthetic gene delivery systems; Schaffer. Inside of the cell: Histidine containing peptides and polypeptides as nucleic acid vectors; Midoux. Intracellular targets for DNA delivery: nuclei and mitochondria; Torchilin. III: Targeted DNA Delivery. Immune cells: Mannose receptor-mediated gene delivery into antigen presenting dendritic cells; Zenke. Muscle: Electro-gene-transfer: a new approach for muscle gene delivery; Toniatti. Tumor: Tumor-targeted gene transfer with DNA polyplexes; Wagner. IV: Novel DNA Delivery Systems. Light: Photochemical transfection: a technology for efficient light directed gene delivery; Hogset. Ultrasound: Sonoportation: mechanical DNA delivery by ultrasonic cavitation; Miller, Greenleaf.

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