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Full Description
Concentrating on the aspects of protein function that are common to the majority of proteins, this collection of methods is brought together for reseachers who are without access to expensive equipment. Using these protocols researchers will be able to get information about the functional properties of any protein. A companion volume, "Protein Structure: A Practical Approach" also edited by Thomas Creighton, provides the methods necessary for the study of protein strucutre.
Contents
Minizing protein in activation; ligand-protein binding affinities; ligand blotting; affinity labelling; cross-linking of protein subunits and ligands by the introduction of disulphide bonds; determining the roles of subunits in protein function; analysis of sequence-specific DNA-binding proteins; identification and purification of sequence-specific DNA-binding proteins; chemical characterization of functional groups in proteins by competitive labelling; chemical modification; site-directed mutagenesis, based on the phosphorothioate approach.
