X-Ray Structure Analysis of Bio-Macromolecular Crystals : Practical Methods and Basic Theory (International Union of Crystallography Texts on Crystallography)

Takenaka, Akio/ Kumasaka, Takashi/ Chatake, Toshiyuki

Oxford University Press（2026/06発売）

• 製本 Hardcover:ハードカバー版／ページ数 240 p.
• 言語 ENG
• 商品コード 9780198933595
• DDC分類 548.8

Full Description

Since the late 19th century, X-ray structural studies have forged ahead through technological progress and the creative ingenuity of researchers. As high intensity X-ray sources and increased speed and sensitivity of the detectors have emerged, it's now possible to image the atomic structure of complex bio-macromolecules, the orbital electron density of small molecules, and to even follow the dynamical behavior of bio-macromolecules.

Textbooks from which junior researchers can grasp the current art have not kept pace with the technological developments and diversification of the field. X-ray studies of bio-macromolecules require a broad understanding of the theory and practical methodologies. A project can include isolating, crystalizing, and adjudicating suitable samples, obtaining and analysing the X-ray data, and finally publishing the results in a standard format. Early decisions in a project can become pitfalls that swallow the unwary. It is increasingly essential to become adept at managing both proteins and nucleic acids. Whereas software is available to help in the critical problem of resolving the phase, expedience often encourages treating such tools as a black-box. The aim here is to help navigate the researcher through these multifarious issues.

The book is intended to serve as a "companion" to experimentalists. It is divided into a general overview of X-ray structural analysis techniques (chapters 1-2) and a detailed discussion (chapters 3-16) that begins with the preparation of proteins and (uniquely) nucleic acids and ends with publishing the results and is organized to lead the reader through the whole process.

Contents

1: Looking into the world of bio-macromolecules
2: Publication of structural analysis
3: Preparation of bio-macromolecules
4: Crystallization of bio-macromolecules
5: Symmetry of crystals
6: X-ray scattering and diffraction
7: Diffraction data collection and computer processing
8: Characteristics of structural factor
9: Phase Problem
10: Structural modeling and phase improvement
11: Structural refinements
12: Maximum likelihood estimation method and crystal structure analysis
13: Validation of analyzed structure
14: Deposition of analyzed structures into Protein Data Bank (PDB)
15: Software for structural analysis
16: Future prospects
17: Appendix