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Full Description
Environmental DNA (eDNA) refers to DNA that can be extracted from environmental samples (such as soil, water, feces, or air) without the prior isolation of any target organism. The analysis of environmental DNA has the potential of providing high-throughput information on taxa and functional genes in a given environment, and is easily amenable to the study of both aquatic and terrestrial ecosystems. It can provide an understanding of past or present biological communities as well as their trophic relationships, and can thus offer useful insights into ecosystem functioning. There is now a rapidly-growing interest amongst biologists in applying analysis of environmental DNA to their own research. However, good practices and protocols dealing with environmental DNA are currently widely dispersed across numerous papers, with many of them presenting only preliminary results and using a diversity of methods. In this context, the principal objective of this practical handbook is to provide biologists (both students and researchers) with the scientific background necessary to assist with the understanding and implementation of best practices and analyses based on environmental DNA.
Contents
1: Introduction to environmental DNA (eDNA)
2: DNA metabarcode choice and design
3: Reference databases
4: Sampling
5: DNA extraction
6: DNA amplification and multiplexing
7: DNA sequencing
8: DNA metabarcoding data analysis
9: Single-species detection
10: Environmental DNA for functional diversity
11: Some early landmark studies
12: Freshwater ecosystems
13: Marine environments
14: Terrestrial ecosystems
15: Palaeoenvironments
16: Host-associated microbiota
17: Diet analysis
18: Analysis of bulk samples
19: The future of eDNA metabarcoding
