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基本説明
ＥＳ細胞操作の樹立への歴史、最新方法を総合的にまとめたテキスト。
Contents - 1 Martin J Evans: Introduction; 2 Frances A. Brook: Procedures for deriving ES cell lines from the mouse; 3 Yoko Kato and Yukio Tsunoda: Production of ES cell-derived mice; 4 Grant R. MacGregor, Wei Wei Fan, Katrina G. Waymire, and Douglas C. Wallace: Generating animal models of human mitochondrial genetic disease using mouse ES cells; 5 Michael V. Wiles and Gabriele Proetzel: Controlling the differentiation of mouse ES cells in vitro; 6 Yelena S. Tarasova, Daniel R. Riordon, Kirill V. Tarasov and Kenneth R. Boheler: In vitro differentiation of mouse ES cells into muscle cells; 7 Osamu Ohneda and Masayuki Yamamoto: In vitro differentiation of mouse ES cells into haematopoietic cells; and more.
Full Description
The groundbreaking isolation of embryonic stem cells (or 'ES cells') of the mouse in the early 1980s triggered a sustained expansion of global research into their exploitation. This led to the routine genetic engineering of the mouse and revolutionised our understanding of biological processes in the context of the whole animal. ES cell biology remains a crucial and growing area of research with far-reaching implications for developmental and comparative biology aswell as for human health. This book serves as a primer to ES cells, their derivation and experimental manipulation. It contains a broad compendium of methods of direct relevance to both graduate students and specialist researchers. An introductory chapter by the principle originator of ES cell research outlines the fundamentals and charts the development of the field. This is followed by comprehensive coverage of state-of-the art techniques for ES cell manipulation, with the mouse as the experimental paradigm, and byrecent innovations with ES cells from human and non-human primates. ES cell-based therapies for otherwise intractable diseases are now being developed with the present challenge to control ES cell growth and differentiation for applications such as cell transplantation - a recurrent theme in this book.As a volume in the Practical Approach Series, the emphasis is on current methods from recognised experts.
Contents
Preface ; 1 Introduction ; 2. Procedures for deriving ES cell lines from the mouse ; 3. Production of ES cell-derived mice ; 4. Generating animal models of human mitochondrial genetic disease using mouse ES cells ; 5. Controlling the differentiation of mouse ES cells in vitro ; 6. In vitro differentiation of mouse ES cells into muscle cells ; 7. In vitro differentiation of mouse ES cells into haematopoietic cells ; 8. Lineage selection and transplantation of mouse ES cell-derived neural precursors ; 9. In vitro differentiation of mouse ES cells into pancreatic and hepatic cells ; 10. Isolation and characterisation of human ES cells ; 11. Differentiation of human ES cells ; 12. ES cell lines from the cynomolgus monkey (Macaca fascicularis) ; Index
