Laboratory Experiments in Microbiology （12TH Spiral）

Johnson, Ted/ Case, Christine

Pearson（2018/01発売）

• ページ数 512 p.
• 言語 ENG
• 商品コード 9780134605203
• DDC分類 579.078

Full Description

For general microbiology laboratory courses

Laboratory Experiments in Microbiology features 57 thoroughly class-tested and easily customizable exercises that teach basic microbiology techniques and applications. The manual provides comprehensive coverage of every area of microbiology across diverse disciplines, including the biological sciences, allied health sciences, agriculture, environmental science, nutrition, pharmacy, and various pre-professional programs. The lab manual is the perfect companion to Tortora/Funke/Case's Microbiology: An Introduction, 13th Edition or any introductory microbiology text.

The 12th Edition of Laboratory Experiments in Microbiology is easier than ever to navigate and more visually effective with new icons indicating when an exercise addresses the human or environmental microbiome, is investigative, or addresses an ASM guideline. New ASM Thinking Skills outline the steps that help develop laboratory thinking skills. Pre-lab quizzes in MasteringTM Microbiology ensure students arrive prepared for each lab, and activities such as Lab and Lecture: Putting It All Together help students see how lab and lecture are integrated.

Contents

PART ONE MICROSCOPY

Use and Care of the Microscope
Examination of Living Microorganisms

PART TWO HANDLING BACTERIA

Microbes in the Environment
Transfer of Bacteria: Aseptic Technique

PART THREE STAINING METHODS

Preparation of Smears and Simple Staining
Negative Staining
Gram Staining
Acid-Fast Staining
Structural Stains (Endospore, Capsule, and Flagella)
Morphological Unknown

PART FOUR CULTIVATION OF BACTERIA

Isolation of Bacteria by Dilution Techniques
Special Media for Isolating Bacteria

PART FIVE MICROBIAL METABOLISM

Carbohydrate Catabolism
Fermentation
Protein Catabolism, Part 1
Protein Catabolism, Part 2
Respiration
Unknown Identification and Bergey's Manual

PART SIX MICROBIAL GROWTH

Oxygen and the Growth of Bacteria
Determination of a Bacterial Growth Curve: The Role of Temperature
Biofilms

PART SEVEN CONTROL OF MICROBIAL GROWTH

Physical Methods of Control: Heat
Physical Methods of Control: Ultraviolet Radiation
Chemical Methods of Control: Disinfectants and Antiseptics
Chemical Methods of Control: Antimicrobial Drugs
Effectiveness of Hand Scrubbing

PART EIGHT MICROBIAL GENETICS

Regulation of Gene Expression
Isolation of Bacterial Mutants
Transformation of Bacteria
DNA Fingerprinting
Genetic Engineering
Ames Test for Detecting Possible Chemical Carcinogens

PART NINE THE MICROBIAL WORLD

Fungi: Yeasts and Molds
Phototrophs: Algae and Cyanobacteria
Protozoa
Parasitic Helminths

PART TEN VIRUSES

Isolation and Titration of Bacteriophages
Plant Viruses

PART ELEVEN INTERACTION OF MICROBE AND HOST

Epidemiology
Koch's Postulate

PART TWELVE IMMUNOLOGY

Innate Immunity
Agglutination Reactions: Slide Agglutination
Agglutination Reactions: Microtiter Agglutination
ELISA Technique

PART THIRTEEN MICRORGANISMS AND DISEASE

Bacteria of the Skin
Bacteria of the Respiratory Tract
Bacteria of the Mouth
Bacteria of the Gastrointestinal Tract
Bacteria of the Urogenital Trac
Identification of an Unknown from a Clinical Sample
Rapid Identification Methods

PART FOURTEEN MICROBIOLOGY AND THE ENVIRONMENT

Microbes in Water: Multiple-Tube Technique
Microbes in Water: Membrane Filter Technique
Microbes in Food: Contamination
Microbes Used in the Production of Foods
Microbes in Soil: The Nitrogen and Sulfur Cycles
Microbes in Soil: Bioremediation

APPENDICES

A. Pipetting
B. Dilution Techniques and Calculations
C. Use of the Spectrophotometer
D. Graphing
E. Use of the Dissecting Microscope
F. Use of the Membrane Filter
G. Electrophoresis
H. Keys to Bacteria