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Full Description
Plant Cell Biology, volume 160 in "Methods in Cell Biology", includes chapters on modern experimental procedures and applications developed for research in the broad area of plant cell biology. Topics covered in this volume include techniques for imaging and analyzing membrane dynamics and movement across membranes; cell wall composition, structure and mechanics; cytoskeleton dynamics and organization; cell development; ion channel physiology; cell mechanics; and methods related to quantifying cell morphogenesis.
Contents
1. Imaging secretory events in plant cells
Chunhua Zhang
2. 3D electron microscopy of plant membrane systems
Marisa Otegui
3. Methods for studying protein targeting to and within chloroplasts
Steve Theg
4. Single channel measurements of plant mechanosensitive ion channels
Elizabeth Haswell
5. Quantitative analysis of cytoplasmic streaming
Andreas Nebenführ
6. Imaging movement through plasmodesmata
Jung-Youn Lee
7. Biochemical analysis of cell wall polymers
Jenny C. Mortimer
8. Expression, biochemistry and structure of plant glycosyltransferases
Breeanna Rae Urbanowicz
9. Studying cell wall mechanics using atomic force microscopy
Sarah Robinson
10. Staining and automated image analysis of callose in the plant cell wall
Antje Heese
11. Tracking of cellulose synthase complexes
Charles Anderson
12. Glycosylation of cell wall proteins
Cora Macalister
13. Super-resolution imaging of microtubules in Medicago sativa
Jozef Šamaj
14. Live imaging of microtubules in petal conical cells
Deshu Lin
15. Purification of plant tubulins for in vitro studies
Takashi Hashimoto
16. Kymograph-based analysis of microtubule dynamics
Ram Dixit
17. Simultaneous measurement of turgor pressure and cell wall elasticity in growing pollen tubes
Ueli Grossniklaus
18. Methods for live cell imaging of pollen biology
Leonor Boavida
19. Morphological analysis of Arabidopsis sepals
Lilan Hong
20. Construction and use of a tensile device for onion epidermis
Anja Geitmann
21. User-friendly assessment of pavement cell shape features with PaCeQuant: novel functions and tools
Katharina Bürstenbinder
22. Imaging pavement cell development
Arun Sampathkumar
23. Microfluidics systems for plant root imaging and analysis
Guido Grossmann and Marjorie Guichard
24. Live imaging and morphometry of roots
Ross Sozzani
25. Correlative electron and light microscopy of plant cells
Jessica Marion
