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Full Description
Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation, Volume 639, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this new release include Fluorogenic detection of protein aggregates in live cells using the AggTag method, Synthesis and Application of Ratiometric Probes for Hydrogen Peroxide Detection, Chemical Tools for Multicolor Protein FRET with Tryptophan, Fluorescing Isofunctional Ribonucleosides for Adenosine Deaminase Activity and Inhibition, Temporal profiling establishes a dynamic S-palmitoylation cycle, Solvation-guided design of fluorescent probes for discrimination of amyloids, and much more.
Contents
Fluorogenic detection of protein aggregates in live cells using the AggTag method
Kwan Ho Jung and Xin Zhang
Synthesis and application of a ratiometric probe for hydrogen peroxide
Ruwen Yin, Yuan Fang, Xinqi Zhou and Cliff I. Stains
Protein labeling for FRET with methoxycoumarin and acridonylalanine
Chloe M. Jones, Yarra Venkatesh and E. James Petersson
Ascertaining the activity and inhibition of adenosine deaminase via fluorescence-based assays
Paul T. Ludford III and Yitzhak Tor
Method to discriminate amyloids using fluorescent probes
Kristine L. Teppang, Rachel S. Ehrlich and Jerry Yang
Chemical modulation of circadian rhythms and assessment of cellular behavior via indirubin and derivatives
Hui-Hsien Lin, Kelly L. Robertson, Sujeewa S. Lellupitiyage Don, Stephanie R. Taylor and Michelle E. Farkas
Methods for analysis of near-infrared (NIR) quenched-fluorescent contrast agents in mouse models of cancer
John C. Widen, Martina Tholen, Joshua J. Yim and Matthew Bogyo
Acid-brightening fluorescent protein (abFP) for imaging acidic vesicles and organelles
Nanxi Wang and Lei Wang
4-Cyanoindole-based fluorophores for biological spectroscopy and microscopy
Arusha Acharyya, Ismail A. Ahmed and Feng Gai
Dissecting contributions of catalytic and reader domains in regulation of histone demethylation
Nektaria Petronikolou, James E. Longbotham and Danica Galonić Fujimori
Ortho-Phthalaldehyde (OPA)-based chemoselective protein bioconjugation and peptide cyclization
Qing Zhang, Yue Zhang and Xuechen Li
Chemical synthesis of di-ubiquitin modified histones for further biochemical studies
Jia-Bin Li, Jun Liang and Changlin Tian
Chemical probes and methods for single-cell detection and quantification of epichaperomes in hematologic malignancies
Swathi Merugu, Sahil Sharma, Justin Kaner, Chander Digwal, Mayumi Sugita, Suhasini Joshi, Tony Taldone, Monica L. Guzman and Gabriela Chiosis
FlICk (fluorescent isoindole crosslinking) for peptide stapling
Mihajlo Todorovic and David M. Perrin
Selective protein N-terminal labeling with N-hydroxysuccinimide esters
Hanjie Jiang, Gabriel D. D'Agostino, Philip A. Cole and Daniel R. Dempsey
Covalent live-cell labeling of proteins using a photoreactive fluorogen
Tewoderos M. Ayele, Steve D. Knutson and Jennifer M. Heemstra
Photoconvertible diazaxanthilidene dyes for live cell imaging
Rachel M. Lackner, Christopher L. Johnny and David M. Chenoweth
No-wash live cell imaging with a photoswitchable near-infrared hCRBPII protein-fluorophore tag
Wei Sheng, James H. Geiger and Babak Borhan
