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Full Description
Tumor Immunology and Immunotherapy - Integrated Methods Part B, Volume 636 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Chapters in this update include Quantification methods of Transforming Growth Factor beta (TGF˗β) activity in the setting of cancer immunotherapy, Decoding cancer cell death-driven immune cell recruitment: An in vivo method for site-of-vaccination analyses, Tracking and interrogating tissue-resident and recruited microglia in brain tumors, Metabolomics and lipidomics of the tumor microenvironment, Monitoring abscopal responses to radiation in mice, and much more.
Contents
1. A comprehensive approach to gene expression profiling in immune cells
Sabrina Carpentier, François Romagné and Eric Vivier
2. DIY: Visualizing the immune landscape of tumors using transcriptome and methylome data
Steven F. Gameiro, Farhad Ghasemi, John W. Barrett, James Koropatnick, Anthony C. Nichols, Joe S. Mymryk and Saman Maleki Vareki
3. Using epigenetic data to estimate immune composition in admixed samples
Ankur Chakravarthy and Daniel De Carvalho
4. Metabolomic approaches to study the tumor microenvironment
Giuseppe Astarita, Suraj Dhungana, Bindesh Shrestha and Evagelia C. Laiakis
5. Immunoscore assay for the immune classification of solid tumors: Technical aspects, improvements and clinical perspectives
Florence Marliot, Lucie Lafontaine and Jérôme Galon
6. Transforming growth factor beta (TGF-β) activity in immune-oncology studies
Gabriel Gallo-Oller, Marianna Di Scala, Fernando Aranda and Javier Dotor
7. Isolation of DNA from exosomes
Sheila Spada, Nils Rudqvist and Erik Wennerberg
8. Decoding cancer cell death-driven immune cell recruitment: An in vivo method for site-of-vaccination analyses
Shentong Fang, Patrizia Agostinis, Petri Salven and Abhishek D. Garg
9. Computational methods in tumor immunology
Bhavneet Bhinder and Olivier Elemento
10. Deconvoluting tumor-infiltrating immune cells from RNA-seq data using quanTIseq
Christina Plattner, Francesca Finotello and Dietmar Rieder
11. Concurrent in situ analysis of point mutations and immune infiltrate in FFPE cancers
Ann-Marie Baker and Trevor A. Graham
12. RNA interference screening methods to identify proliferation determinants and mechanisms of resistance to immune attack
Yong-Wei Zhang, Rochelle E. Nasto, Sandra A. Jablonski, Ilya G. Serebriiskii, Rishi Surana, Joseph Murray, Michael Johnson, Rebecca B. Riggins, Robert Clarke, Erica A. Golemis and
Louis M. Weiner
13. An altered miTRAP method for miRNA affinity purification with its pros and cons
Simon Jasinski-Bergner, Christoforos Vaxevanis, Nadine Heimer, Maria-Filothei Lazaridou, Michael Friedrich and Barbara Seliger
14. Identification of immunomodulatory RNA-binding proteins in tumors
Michael Friedrich, Maria-Filothei Lazaridou, Jette Rahn, Christoforos Vaxevanis, Nadine Heimer, Simon Jasinski-Bergner and Barbara Seliger
15. Techniques for the generation of humanized mouse models for immuno-oncology
Chun I. Yu, Florentina Marches, Te-Chia Wu, Jan Martinek and Karolina Palucka
