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Full Description
Chemical and Synthetic Biology Approaches to Understand Cellular Functions - Part C, Volume 633, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial. This release includes sections on Next generation probes for molecular imaging in cells, Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein, Converting avidin to bind ligands other than biotin, especially steroids, Chemoenzymatic Labeling Strategy, Engineered Siderophores, Small molecules to inhibit bacterial population behavior, NMR tube bioreactor, Small molecule controlled RAS activation system, Small molecule regulated Cas9, the Design and application of synthetic receptors, and much more.
Contents
1. Competitive binding assay for biotin and biotin derivatives, based on avidin and biotin-4-fluorescein
Elke Oberbichler, Maria Wiesauer, Eva Schlögl, Jessica Stangl, Felix Faschinger, Günther Knör, Hermann J. Gruber and Vesa P. Hytönen
2. (Strept)avidin as a template for ligands other than biotin: An overview
Vesa P. Hytönen
3. Engineering siderophores
Sina Rütschlin and Thomas Böttcher
4. Competitive profiling for enzyme inhibitors using chemical probes
Michaela Prothiwa and Thomas Böttcher
5. The NMR tube bioreactor
Alexandra V. Chatzikonstantinou, Antonis Tsiailanis, Ioannis P. Gerothanassis, Haralambos Stamatis, Enrico Ravera, Marco Fragai, Claudio Luchinat, Giacomo Parigi and Andreas G. Tzakos
6. A chemically-controlled system for activating RAS GTPases
Emily M. Dieter and Dustin J. Maly
7. Temporal and rheostatic control of genome editing with a chemically-inducible Cas9
Cindy T. Wei, Dustin J. Maly and Douglas M. Fowler
8. Synthetic receptors to understand and control cellular functions
Hung-Ju Chang and Jerome Bonnet
9. A suite of bioassays to evaluate CREB inhibitors
Bingbing X. Li and Xiangshu Xiao
10. Identification of lamins as the molecular targets of LBL1 using a clickable photoaffinity probe
Xiangshu Xiao and Bingbing X. Li
11. REX technologies for profiling and decoding the electrophile signaling axes mediated by Rosetta Stone proteins
Marcus J. C. Long, Daniel A. Urul and Yimon Aye
12. Building artificial genetic circuits to understand protein function
Louis H. Scott, James C. Mathews, Aleksandra Filipovska and Oliver Rackham
13. Methods for studying human sirtuins with activity-based chemical probes
Song Zheng, Jessica Wohlfahrt, Ian Cohen and Yana Cen
14. Site-directed labeling of β-arrestin with monobromobimane for measuring their interaction with G protein-coupled receptors
Ashish Srivastava, Mithu Baidya, Hemlata Dwivedi-Agnihotri and Arun K. Shukla
15. Reversible biotinylation of purified proteins for measuring protein-protein interactions
Hemlata Dwivedi-Agnihotri, Ashish Srivastava and Arun K. Shukla
