Full Description
Tumor Immunology and Immunotherapy Integrated Methods - Part A, Volume 635 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Specific chapters to this release include Deconvolution of the immunological contexture of mouse tumors with multiplexed immunohistochemistry, High-dimensional multiplexed immunohistochemical characterization of immune contexture in human cancers, Multiplex assay by IHC for melanoma tumor microenvironment evaluation, Characterization of the tumor immune microenvironment by multispectral image analysis of multiplex immunofluorescence images, Phenotyping of immune cells in situ using multispectral imaging quantification, and much more.
Contents
1. High-dimensional multiplexed immunohistochemical characterization of immune contexture in human cancers
Grace Banik, Courtney B. Betts, Shannon M. Liudahl, Shamilene Sivagnanam, Rie Kawashima, Tiziana Cotechini, William Larson, Jeremy Goecks, Sara I. Pai, Daniel R. Clayburgh, Takahiro Tsujikawa and Lisa M. Coussens
2. Multiplex immunohistochemistry assay to evaluate the melanoma tumor microenvironment
Giosuè Scognamiglio, Mariaelena Capone, Domenico Mallardo, Gerardo Botti, Paolo A. Ascierto and Gabriele Madonna
3. Characterization of the immune microenvironment of NSCLC by multispectral analysis of multiplex immunofluorescence images
Michael Surace, Lorenz Rognoni, Jaime Rodriguez-Canales and Keith E. Steele
4. Multiplexed immunohistochemistry for immune cell phenotyping, quantification and spatial distribution in situ
Angela Vasaturo and Jérôme Galon
5. Multiplex immunohistochemistry as a novel tool for the topographic assessment of the bone marrow stem cell niche
Marcus Bauer, Christoforos Vaxevanis, Daniel Bethmann, Chiara Massa, Nikolaos Pazaitis, Claudia Wickenhauser and Barbara Seliger
6. Deconvolution of the immunological contexture of mouse tumors with multiplexed immunohistochemistry
Ileana S. Mauldin, Natasha D. Sheybani, Samuel J. Young, Richard J. Price and Craig L. Slingluff
7. Tracing bone marrow-derived microglia in brain metastatic tumors
Wei Du and Paula D. Bos
8. Monitoring abscopal responses to radiation in mice
Maria Esperanza Rodriguez-Ruiz, Takahiro Yamazaki, Aitziber Buqué, Norma Bloy, Viviane A. O. Silva, Lena Stafford, Ai Sato and Lorenzo Galluzzi
9. Methods to assess anticancer immune responses in orthotopic bladder carcinomas
Randy F. Sweis
10. Characterization of conventional dendritic cell populations in preclinical tumor models using flow cytometry
Erik Wennerberg, Nils Rudqvist and Sheila Spada
11. Quantitative evaluation of tumor-specific T cells in tumors and lymphoid tissues
Kathleen M. Kokolus, Nataša Obermajer and Pawel Kalinski
12. Generation and validation of recombinant herpes simplex type 1 viruses (HSV-1) using CRISPR/Cas9 genetic disruption
Praveen K. Bommareddy, Cole Peters and Howard L. Kaufman
13. Production and use of adeno-associated virus vectors as tools for cancer immunotherapy
Myriam Fernandez-Sendin, Shirley Tenesaca, Marcos Vasquez, Fernando Aranda and Pedro Berraondo
14. Modulation of the tumor microenvironment with an oncolytic adenovirus for effective T-cell therapy and checkpoint inhibition
João Manuel Santos, Riikka Havunen and Akseli Hemminki
15. Genetic modification of oncolytic viruses to enhance antitumor immunity
Maria Eugenia Davola, Alyssa Vito, Jiarun Wei, Nader El-Sayes, Samuel Workenhe and Karen Louise Mossman
16. CRISPR technology for immuno-oncology applications
Jianbin Wang, Vitaly Balan and Francesco Marincola
17. Neoantigen prediction from genomic and transcriptomic data
Sebastiano Battaglia
18. Monitoring antigen cross-presentation with human dendritic cells purified from the blood
Stephane Isnard, Etienne Hatton, Jean-Baptiste Guillerme and Anne Hosmalin
