Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields.
1. An introduction to genome editing CRISPR-Cas systems 2. Evolution and mechanism of CRISPR-Cas9 systems 3. Exploring the potential of CRISPR-Cas9 for removal of human viruses 4. Programmable removal of bacterial pathogens using CRISPR-Cas9 system 5. Targeted genome editing using CRISPR-Cas9 system in fungi 6. CRISPR-Cas9 system for fungi genome engineering towards industrial applications 7. Development and challenges of using CRISPR-Cas9 system in mammalians 8. CRISPR technology for cell line development and engineering 9. Exploring the potential of genome editing CRISPR-Cas9 system in cancer 10. CRISPR-Cas9 for therapy: the challenges and ways to overcome them 11. Engineered Cas9 nucleases 12. The current progress of CRISPR/Cas9 development in plants 13. Fruit crops improvement using CRISPR-Cas9 system 14. CRISPR-Cas9 engineered viral immunity in plants 15. Genome engineering in medicinally important plants using CRISPR/Cas9 tool 16. Genome editing of algal species by CRISPR Cas9 for biofuels 17. Development and use of CRISPR in industrial applications 18. CRISPR interference based gene silencing in bacteria: Advantages and limitations 19. Genome engineering in insects: focus on the CRISPR/Cas9 system 20. Recent Progress of CRISPR- Cas9 in Zebra Fish 21. CRISPR: a revolutionary tool for genome engineering in the protozoan parasites 22. Biosafety, biosecurity, patents, and regulatory issues