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Full Description
Mechanisms of DNA Recombination and Genome Rearrangements: Intersection between Homologous Recombination, DNA Replication and DNA Repair, Volume 601, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field.
Homologous genetic recombination remains the most enigmatic process in DNA metabolism. The molecular machines of recombination preserve the integrity of the genetic material in all organisms and generate genetic diversity in evolution. The same molecular machines that support genetic integrity by orchestrating accurate repair of the most deleterious DNA lesions, however, also promote survival of cancerous cells and emergence of radiation and chemotherapy resistance. This two-volume set offers a comprehensive set of cutting edge methods to study various aspects of homologous recombination and cellular processes that utilize the enzymatic machinery of recombination. The chapters are written by the leading researches and cover a broad range of topics from the basic molecular mechanisms of recombinational proteins and enzymes to emerging cellular techniques and drug discovery efforts.
Contents
1. Using the Flp recombinase to induce site-specific nicks
Lotte Bjergbaek
2. Synthesis from D-loops
Wolf-Dietrich Heyer
3. Bacteriophage T4 system as a model for recombination/replication interface
Scott Morrical
4. Inducing and detecting mitotic DNA synthesis at difficult-to-replicate loci
Ian Hickson
5. Reporter system to study the RNA-DNA recombination
Francesca Storici
6. Assaying recombination and mutagenesis at DNA nicks
Nancy Maizels
7. Recovery of alternative end-joining repair products from Drosophila embryos
Mitch McVey
8. High-throughput analysis of DNA break-induced chromosome rearrangements by amplicon sequencing
Steven Roberts
9. Analysis of mutagenesis associated with recombination
Jim Haber
10. Investigation of break-induced replication in yeast
Anna Malkova
11. Dissecting the dual endo- and exonuclease activity of MRE11 (MRN) with small molecules
John Tainer and Davide Moiani
12. RAD52 inhibition
Ashley Spies
13. Structure-guided, fragment-based drug discovery against RAD51 recombinase: targeting a protein-peptide interface
Thomas Blundell
14. Molecular intermediates of recombination in meiosis (methods, like 2D, and others)
Neil Hunter
15. S1-seq method for mapping DSB resection endpoints
Scott Keney
16. Methods for studying the roles of the Sgs1-Top3-Rmi1 helicase/decatenase complex in meiotic recombination intermediate metabolism
Michael Lichten
17. Live cell imaging of heterochromatin repair in Drosophila cells
Irene Chiolo
18. Analysis of meiotic double-strand break initiation in mammals
Galina Petukhova and Dan Camerini-Otero
