光合成有機体における炭水化物代謝の解析手法 Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms : Plants, Green Algae and Cyanobacteria

Pontis, Horacio G

Academic Press Inc（2016/09発売）

• 製本 Paperback:紙装版/ペーパーバック版／ページ数 250 p.
• 言語 ENG
• 商品コード 9780128033968
• DDC分類 572.564

Full Description

Methods for Analysis of Carbohydrate Metabolism in Photosynthetic Organisms: Plants, Green Algae and Cyanobacteria examines both general and detailed aspects of carbohydrate metabolism in photosynthetic organisms, along with the four main oligosaccharides and each enzymatic reaction that gives birth to them. Chapters include information on how biological active protein is extracted for different cells, determination of enzymatic activity, separation of proteins by different available methods, and descriptions of analytical methods for the determination of various types of carbohydrates in photosynthetic organisms.

The book contains useful protocols for researchers working on the determination of carbohydrate metabolism. The book provides foundational content as well as step-by-step guidance on how to design and conduct an experiment, including what other methodologies could be used if advanced instruments are not readily available.

Contents

Part I. General Analytical Methods1. Determination of carbohydrates metabolism molecules

Part II. Purification and analysis of proteins and carbohydrates2. Preparation of protein extracts3. Protein and carbohydrate separation and purification4. Measurement of enzyme activity5. General introduction to Mass Spectrometry and Nuclear Magnetic Resonance

Part III. Case studies6. Sucrose7. Trehalose8. Raffinose9. Fructans10. Polysaccharides11. Starch 12. Glycogen13. Cellulose14. Sugar phosphates15. Nucleotide sugars