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Full Description
Membrane Proteins - Engineering, Purification and Crystallization, a volume of Methods In Enzymology, encompasses chapters from the leading experts in the area of membrane protein biology. The chapters provide a brief overview of the topics covered and also outline step-by-step protocol for the interested audience. Illustrations and case example images are included wherever appropriate to help the readers understand the schematics and general experimental outlines.
Contents
Section I. Membrane Protein Engineering, Solubilization and Purification
1. Multicolor fluorescence-based screening toward structural analysis of multiprotein membrane complexes
Simon Trowitzsch & Robert Tampé
2. A novel screening approach for optimal and functional fusion of T4 lysozyme in GPCRs
Elizabeth Mathew and Mark E Dumont
3. Membrane preparation and solubilization
Ankita Roy
4. Amphipathic agents for membrane protein study
Aiman Sadaf, Kyung Ho Cho, Bernadette Byrne, Pil Seok Chae
5. Quantification of detergent using colorimetric methods in membrane protein crystallography
Chelsy Prince , Zongchao Jia
6. Solubilization of G Protein-Coupled Receptors: A Convenient Strategy to Explore Lipid-Receptor Interaction
Amitabha Chattopadhyay, Bhagyashree D. Rao and Md. Jafurulla
7. Overexpression, Isolation, Purification and Crystallization of NhaA
Etana Padan and Manish Dwivedi
8. Purification, refolding and crystallization of the outer membrane protein OmpG from Escherichia coli
Stefan Köster, Katharina van Pee, Özkan Yildiz
9. Biophysical Approaches to the Study of LeuT, a Prokaryotic Homolog of Neurotransmitter Sodium Symporters
Satinder K. Singh and Aritra Pal
Section II. Generation and Use of Antibody Fragments Against Membrane Proteins
10. Generation of recombinant antibody fragments for membrane protein crystallization
Syed H. Mir , Claudia Escher, Wei-Chun Kao, Dominic Birth, Christophe Wirth, Carola Hunte
11. Phage Display Selections for Affinity Reagents to Membrane Proteins in Nanodiscs
Pawel K. Dominik, and Anthony A. Kossiakoff
12. Antibody fragments for crystallization, trapping of active conformations and stabilization of G-protein coupled receptors and their signaling complexes
Arun K. Shukla, Charu Gupta, Ashish Srivastava and Deepika Jaiman
Section III. Biophysical Studies of Membrane Proteins
13. Conformational Analysis of G Protein-Coupled Receptor Signaling by Hydrogen/Deuterium Exchange Mass Spectrometry
Sheng Li, Su Youn Lee and Ka Young Chung
14. EPR Studies of Gating Mechanisms in Ion Channels
Sudha Chakrapani
15. Magic-Angle-Spinning Solid-State NMR of Membrane Proteins
Lindsay A Baker, Gert E Folkers, Tessa Sinnige, Klaartje Houben, Mohammed Kaplan, Elwin AJ van der Cruijsen, Marc Baldus
16. Solution NMR Structure Determination of Polytopic α-helical membrane proteins: A guide to spin label paramagnetic relaxation enhancement restraints
Linda Columbus and Brett Kroncke
Section IV. Crystallization of Membrane Proteins
17. Inducing Two-Dimensional Crystallization of Membrane Proteins by Dialysis for Electron Crystallography
Yusuf M. Uddin and Ingeborg Schmidt-Krey
18. Crystallization of Membrane Proteins by Vapor Diffusion
Jared A. Delmar, Jani Reddy Bolla, Chih-Chia Su, and Edward W. Yu
19. An empirical approach to bicelle crystallization
Sandra Poulos, Jacob L. W. Morgan, Jochen Zimmer, Salem Faham
20. Fluorescence Recovery After Photobleaching in Lipidic Cubic Phase (LCP-FRAP): A pre-crystallization assay for membrane proteins
Gustavo Fenalti, Enrique E. Abola, Chong Wang, Beili Wu, Vadim Cherezov
21. Crystallization of Proteins from Crude Bovine Rod Outer Segments
Bo Y Baker, Sahil Gulati, Wuxian Shi, Benlian Wang, Phoebe L. Stewart, and Krzysztof Palczewski
22. Crystallization of Photosystem II for Time Resolved Structural Studies Using an X-ray Free Electron Laser
Jesse Coe, Christopher Kupitz, Shibom Basu, Chelsie E. Conrad, Shatabdi Roy-Chowdhury, Raimund Fromme, Petra Fromme
Section V. Computational Approaches to Understand Membrane Proteins
23. Major Intrinsic Protein Superfamily: Channels with Unique Structural Features and Diverse Selectivity Filters
Ravi Kumar Verma, Anjali Bansal Gupta and Ramasubbu Sankararamakrishnan
24. Comparative Sequence-Function Analysis of the Major Facilitator Superfamily: the 'Mix-and-Match' Method
M. Gregor Madej
25. Elucidating Ligand-Modulated Conformational Landscape of GPCRs Using Cloud-computing Approaches
Diwakar Shukla, Morgan Lawrenz and Vijay S. Pande
