Full Description
These new volumes of Methods in Enzymology (554 and 555) on Hydrogen Sulfide Signaling continue the legacy established by previous volumes on another gasotransmitter, nitric oxide (Methods in Enzymology volumes 359, 396, 440, and 441), with quality chapters authored by leaders in the field of hydrogen sulfide research. These volumes of Methods in Enzymology were designed as a compendium for hydrogen sulfide detection methods, the pharmacological activity of hydrogen sulfide donors, the redox biochemistry of hydrogen sulfide and its metabolism in mammalian tissues, the mechanisms inherent in hydrogen sulfide cell signaling and transcriptional pathways, and cell signaling in specific systems, such as cardiovascular and nervous system as well as its function in inflammatory responses. Two chapters are also devoted to hydrogen sulfide in plants and a newcomer, molecular hydrogen, its function as a novel antioxidant.
Contents
THE REDOX BIOCHEMISTRY OF HYDROGEN SULFIDE
1. Investigating the Role of H2S in 4-HNE Scavenging
Hilde Laggner and Bernhard M.K. Gmeiner
2. Inhalation Exposure Model of Hydrogen Sulfide (H2S)-Induced Hypometabolism in the Male Sprague-Dawley Rats
Asaf Stein, David W. Kraus, Jeannette E. Doeller and Shannon M. Bailey
MECHANISMS OF H2S CELL SIGNALING AND TRANSCRIPTIONAL PATHWAYS
3. Use of the "Tag-Switch” Method for the Detection of Protein S-Sulfhydration
Chung-Min Park, Igor Macinkovic, Milos R. Filipovic, Ming Xian
4. Real-Time Assays for Monitoring the Influence of Sulfide and Sulfane Sulfur Species on Protein Thiol Redox States
Romy Greiner and Tobias P. Dick
5. Sulfhydration of H2S Protein Targets
Bindu D. Paul and Solomon H. Snyder
H2S IN CELL SIGNALING IN THE CARDIOVASCULAR AND NERVOUS SYSTEM AND INFLAMMATORY PROCESSES
6. Intravital Microscopic Methods to Evaluate Anti-inflammatory Effects and Signaling Mechanisms Evoked by Hydrogen Sulfide
Mozow Y. Zuidema and Ronald J. Korthuis
7. Attenuation of Inflammatory Responses by Hydrogen Sulfide (H2S) in Ischemia / Reperfusion Injury
Neel R. Sodha and Frank W. Sellke
8. CD47-dependent Regulation of H2S Biosynthesis and Signaling in T Cells
Sukhbir Kaur, Anthony L. Schwartz, Thomas W. Miller and David D. Roberts
9. Anti-Inflammatory and Cytoprotective Properties of Hydrogen Sulfide
Burcu Gemici and & John L. Wallace
10. H2S and Substance P in Inflammation
Madhav Bhatia
11. Role of Hydrogen Sulfide (H2S) in Brain Synaptic Remodeling
Pradip Kumar Kamat, Anuradha Kalani and Neetu Tyagi
H2S IN PLANTS
12. Detection of Thiol Modifications by Hydrogen Sulfide
Williams, E., Pead, S., Whiteman, M., Wood, M.E., Wilson, I.D., Ladomery, M.R., Teklic, T., Lisjak, M. and Hancock, J.T.
13. Analysis of Some Enzymes Activities of Hydrogen Sulfide Metablism in Plants
Zhong-Guang Li
14. Sulfide Detoxification in Plant Mitochondria
Hannah Birke, Tatjana M. Hildebrandt, Markus Wirtz and Rüdiger Hell
MOLECULAR HYDROGEN
15. Molecular Hydrogen as a Novel Antioxidant: Overview of the Advantages of Hydrogen for Medical Applications
Shigeo Ohta
