神経科学における基礎分子プロトコル Basic Molecular Protocols in Neuroscience: Tips, Tricks, and Pitfalls

Corthell, John T.

Academic Press Inc（2014/04発売）

• 製本 Paperback:紙装版/ペーパーバック版／ページ数 124 p.
• 言語 ENG
• 商品コード 9780128014615
• DDC分類 612.8

Full Description

Basic Neuroscience Protocols: Tips, Tricks, and Pitfalls contains explanatory sections that describe the techniques and what each technique really tells the researcher on a scientific level. These explanations describe relevant controls, troubleshooting, and reaction components for some of the most widely used neuroscience protocols that remain difficult for many neuroscientists to implement successfully. Having this additional information will help researchers ensure that their experiments work the first time, and will also minimize the time spent working on a technique only to discover that the problem was them, and not their materials.

Contents

Introduction

General Notes

DNA and RNA Extraction Protocols

Agarose Gell Electrophoresis

Reverse Transcription (RT) and Polymerase Chain Reaction (PCR)

Antibodies and Titrations

Protein Extraction

Immunoblotting (Western Blot)

Immunoprecipitation

Perfusion and Immersion Fixation

Immunohistochemistry

In situ Hybridization

References