Full Description
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers G protein coupled receptors, and includes chapters on such topics as GPCR modelling, interactions with other molecules, virtual screening and GPCR activation.
Contents
GPCR Modeling
The Protein Local Optimization Program and G-Protein Coupled Receptors: Loop Restoration and Applications to Homology Modeling
Modeling Active GPCR Conformations
Molecular Modeling of Adenosine Receptors
Predicting G Protein-Coupled Receptors Families using Different Physiochemical Properties and Pseudo Amino Acid Composition
Computational Design and Experimental Characterization of GPCR Segment Models
GPCR Interactions with Other Molecules and Cross-Talk
G Protein-Coupled Receptor Interaction with Small GTPases
Quantification of Receptor Tyrosine Kinase Activation and Transactivation by G Protein Coupled Receptors Using Spatial Intensity Distribution Analysis (SpIDA)
NMR Methods for Detection of Small Molecule Binding to RGS4
Group II Activators of G-protein Signaling: Monitoring the Interaction of Ga with the G-Protein Regulatory Motif in the Intact Cell
Homogeneous Time-Resolved Fluorescence Assay to Probe Folded G Protein-Coupled Receptors
cAMP Biosensors Applied in Molecular Pharmacological Studies of G Protein-Coupled Receptors
Rhodopsin-Lipid Interactions Studied by NMR
GPCR Bias
Biasing the Parathyroid Hormone Receptor: Relating In Vitro Ligand Efficacy to In Vivo Biological Activity
Virtual Screening
Application of Monte Carlo-based Receptor-Ensemble Docking to Virtual Screening for GPCR Ligands
From Heptahelical Bundle to Hits from the Haystack: Structure-Based Virtual Screening for GPCR Ligands
GPCR Activation
Kinetics and Dynamics in the G-Protein Coupled Receptor Signaling Cascade
Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy of G Protein-Coupled Receptors
Post-Translation Modification of G Protein-Coupled Receptor in Relationship to Biased Agonism
Protein S-Nitrosylation Measurement
