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Full Description
Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone.
Contents
Single Molecule FRET Microscopy
Bioluminescent Imaging of Gene Expressions
Confocal Imaging
Electron Microscopy of Bone
Immunostaining: Detection of Signaling Protein Location in Tissues, Cells and Subcellular Compartments
Bimolecular Fluorescence Complementation (BiFC) Analysis of Protein-Protein Interactions
Histochemistry
Live Imaging
Click Labeling for Single-Molecule FRET
High Throughput Methods for the Detection of Protein Over Expression Using Fluorescent Markers
Correlation Microscopy
Stereology of the Lung
Formation and Preparation of Cellular Spheroids for High-Content Assays Based on Epifluorescence and Laser Light Sheet Fluorescent Microscopies.
Optimising Fluorochrome Excitation and Detection for Intravital 2-Photon Microscopy
Cryomicroscopy
In situ Hybridization
Imaging RNA