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Full Description
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as methods for studying ciliary polarity in Xenopus, analysis of signaling pathways in mammalian spermatozoa, and biochemical and physiological analysis of axonemal dyneins.
Contents
Methods for studying ciliary polarity in Xenopus
Analysis of signaling pathways in mammalian spermatozoa
Analysis of tubulin modification in zebrafish cilia
Analysis of microtubule end-binding proteins in cilia
Small molecule screens using Chlamydomonas flagella
Biochemical and Physiological analysis of axonemal dyneins
Analysis of ciliary assembly and function in Planaria
Reconstitution of flagellar sliding
Comparative genomic analysis of ciliary genes
Analysis of ciliogenesis in Tetrahymena
Measuring the regulation of dynein activity during flagellar motility
Centrosome isolation and proteomics
Probing ciliogenesis using micropatterned substrates
3D structural analysis of flagella/cilia by electron cryo-tomography
Visualizing IFT in mammalian cilia
Ciliogenesis in multiciliated epithelial cells in culture
Methods for analysis of PKD-containing urinary exosomes
Analysis of Interactions between IFT Proteins
Electron microscopy of Intraflagellar Transport
Using the SNAP tag to measure ciliary trafficking