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基本説明
The second of 2 volumes.
Full Description
This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The second of 3 volumes covering Natural product biosynthesis by microorganisms and plants.
Contents
In vivo production of thiopeptide variants
Microviridin biosynthesis
Cyclotide isolation and characterization
Ribosomally-encoded cyclic peptide toxins from mushrooms
The Pictet-Spengler mechanism involved in the biosynthesis of tetrahydroisoquinoline antitumor antibiotics: novel function for a non-ribosomal peptide synthetase
Discovery and biosynthesis of phosphonate and phosphinate natural products
RlmN and AtsB as models for the overproduction and characterization of radical SAM proteins
Fe(II)-dependent, uridine-5'-monophosphate α-ketoglutarate dioxygenases in the synthesis of 5'-modified nucleosides
Heme-dependent Tailoring Enzymes in Natural Product Biosynthesis: Spectroscopic Characterisation of the L-tryptophan-nitrating Cytochrome P450 TxtE
Oxidative Tailoring Reactions Catalysed by Non-heme Iron-dependent Enzymes: Streptorubin B Biosynthesis as an Example
The rare fluorinated natural products and biotechnological prospects for fluorine enzymology
Enzymatic chlorination and bromination
Prenyltransferases of the dimethylallyltryptophan synthase superfamily
Serine carboxypeptidase-like acyltransferases from plants
4-Methylideneimidazole-5-one-containing aminomutases in enediyne biosynthesis
Tailoring enzymes acting on carrier protein-tethered substrates in natural product biosynthesis
High-throughput colorimetric assays for nucleotide sugar formation and glycosyl transfer