Full Description
This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. This volume covers G protein coupled receptors and includes chapters on such topics as post-translation modification of GPCR in relationship to biased agonism, structure-based virtual screening, and GPCR oligomerization in the brain.
Contents
G Protein Coupled Receptors: Techniques for Probing Structure
Comformational Ensemble View of G Protein-Coupled Receptors and The Effect of Mutations and Ligand Binding
Structural Evolution of G-Protein-Coupled Receptors: A Sequence Space Approach
Directed Evolution of G Protein-Coupled Receptors for High Functional Expression and Detergent Stability
The Role of Hydrophobic Amino Acids in the Structure and Function of the Rhodopsin Family of G Protein-Coupled Receptors
Structure of β-Adrenergic Receptors
Advances in Methods to Characterize Ligand-Induced Ionic Lock and Rotamer Toggle Molecular Switch in G-Protein Coupled Receptors
Crystallogenesis of Adenosine A2A Receptor - T4 Lysozyme Fusion Protein: A Practical Route for the Structure
Probing GPCR Structure: Adenosine and P2Y Nucleotide Receptors
Strategies for Studying the Ligand Binding Site of GPCRs: Photoaffinity Labeling of the VPAC1 Receptor, a Prototype of Class B GPCRs
Expression of Mammalian G Protein-Coupled Receptors in Caenorhabditis Elegans
Expression, Purification and Structural Analysis of Intracellular C-Termini from Metabotropic Glutamate Receptors
Unnatural Amino Acid Mutagenesis of GPCRs Using Amber Codon Suppression and Bioorthogonal Labeling
Mapping a Ligand Binding Site Using Genetically-Encoded Photoactivatable Crosslinkers
Alternative mRNA Splicing of G Protein-Coulped Receptors
Functional Residues of the CB1 Cannabinoid Receptor
