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基本説明
Provides state-of-the-art updates on methods and protocols dealing with the detection, isolation and characterization of macromolecules and their hosting organisms that facilitate nitrification and related processes in the nitrogen cycle as well as the challenges of doing so in very diverse environments.
Full Description
The global nitrogen cycle is the one most impacted by mankind. The past decade has changed our view on many aspects of the microbial biogeochemical cycles, including the global nitrogen cycle, which is mainly due to tremendous advances in methods, techniques and approaches. Many novel processes and the molecular inventory and organisms that facilitate them have been discovered only within the last 5 to 10 years, and the process is in progress. Research on Nitrification and Related Processes, Part B provides state-of-the-art updates on methods and protocols dealing with the detection, isolation and characterization of macromolecules and their hosting organisms that facilitate nitrification and related processes in the nitrogen cycle as well as the challenges of doing so in very diverse environments.
Contents
Part 1: Process Measurements in Natural Systems1. Strategies to determine diversity, growth and activity of ammonia oxidising archaea in soil2. Profiles as bio-indicators in polluted coastal marine environments and non-polluted ocean water and sediments3. Molecular and Stable Isotope Methods to Detect and Measure Anaerobic Ammonium Oxidation (anammox) in Aquatic Ecosystems4. Nitrogen Mineralization and Assimilation at Millimeter Scales5. Measurement of carbon dioxide, methane, nitrous oxide and water potential in soil ecosystems6. Source determination of nitrous oxide based on nitrogen and oxygen isotope tracing: dealing with oxygen exchangePart 2: Process Measurements in Biofilm Communities7. Structure, function, and eco-physiological interactions among community members in complex multispecies biofilms8. In Situ Techniques and Digital Image Analysis Methods for Quantifying Spatial Localization Patterns of Nitrifiers and Other Microorganisms in Biofilm and Flocs9. Investigating Nitrosomonas europaea stress biomarkers in batch, continuous culture and biofilm reactors10. Nitrification of Raw or Used Water Using Expanded Bed Biofilm Reactor Technology11. Ammonia Oxidizing Bacteria In WastewaterPart 3: Genomics, Metagenomics and Transcriptomics12. Genomics for key players in the N cycle: From Guinea pigs to the next frontier13. Preparation of high-molecular-weight DNA and metagenomic libraries from soils and hot springs14. Characterizing bacterial gene expression in nitrogen cycle metabolism with RT-qPCR15. The utility of functional gene arrays for assessing community composition, relative abundance and distribution of ammonia-oxidizing bacteria and archaeaPart 4: Biochemistry & Proteomics16. Structure and Function of Formate-Dependent Cytochrome c Nitrite Reductase, NrfA17. Structure and function of Multicopper Oxidase Enzymes of Ammonia-Oxidizing Bacteria18. Assessing variability in gel-based proteomic analysis of Nitrosomonas europaea19. Nitrogen Metabolism and Kinetics of Marine Archaea and other oligotrophs
