- ホーム
- > 洋書
- > 英文書
- > Science / Mathematics
基本説明
長年の試行錯誤を重ね、確立した実験プロトコルを精選収録したガイドの改訂版。新版では、30%の新規内容を収録。
Presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. 30% new material in this edition inlcudes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and expanded glossary. Prev. Ed: 2005.
Full Description
This is the fourth edition of the successful laboratory guide which has translated the rich story of riboneucleic acid for over fifteen years. RNA Methodologies 4e presents the latest collection of tested laboratory protocols for the isolation and characterization of eukaryotic and prokaryotic RNA with greater emphasis on transcript profiling, including quantification issues and elucidation of alternative transcription start sites. Collectively the chapters work together providing analysis with clear take-home lessons to assist researchers to understand RNA and to optimize time at the bench. The abundant use of flow charts, tables and graphs are especially helpful in the planning and implementation phases of a project and facilitate learning. 30% new material in this edition includes the addition of RNA isolation protocols including RNA isolation from tissue, expansion of PCR optimization analysis and RNA interference sections, the introduction of a new chapter dealing with the molecular biology of plants, and an expanded glossary.
Contents
**Fully revised with five new chapters, a new appendix, and nine chapters with major expansions** CHAPTER 1: RNA and the Cellular Biochemistry RevisitedCHAPTER 2: RNA Isolation StrategiesCHAPTER 3: The Truth about TissuesCHAPTER 4: Going Green: RNA and the Molecular Biology of Plants (NEW)CHAPTER 5: Isolation of Polyadenylated RNACHAPTER 6: Quality Control for RNA Preparations (EXPANDED)CHAPTER 7: Resilient RibonucleasesCHAPTER 8: Stringency: Conditions that Influence Nucleic Acid Structure (EXPANDED)CHAPTER 9: Electrophoresis of RNA (EXPANDED)CHAPTER 10: Photodocumentation and Image Analysis (EXPANDED)CHAPTER 11: Northern AnalysisCHAPTER 12: Nucleic Acid Probe TechnologyCHAPTER 13: Practical Nucleic Acid HybridizationCHAPTER 14: Principles of Detection (EXPANDED)CHAPTER 15: Quantification of Specific mRNAs by Nuclease Protection CHAPTER 16: Analysis of Nuclear RNA (EXPANDED)CHAPTER 17: cDNA Synthesis (EXPANDED)CHAPTER 18: RT-PCR (NEW)CHAPTER 19: Quantitative PCR Techniques CHAPTER 20: Functional Genomics and Transcript Profiling (NEW)CHAPTER 21: High-Throughput Analysis of Gene Expression CHAPTER 22: Non-Array Methods for Global Analysis of Gene Expression (NEW)CHAPTER 23: RNA Interference: Targeted Gene Silencing (EXPANDED)CHAPTER 24: Genomes, Transcriptomes, Proteomes, and BioinformaticsCHAPTER 25: An RNA ParadigmAPPENDIX A: Maintaining Complete and Accurate RecordsAPPENDIX B: Converting Mass to Moles (NEW)APPENDIX C: Useful Stock Solutions for the Molecular BiologistAPPENDIX D: Phenol PreparationAPPENDIX E: Disposal of Ethidium Bromide and SYBR Green SolutionsAPPENDIX F: DNase I Removal of DNA From An RNA SampleAPPENDIX G: DNase Incubation to Remove RNA from a DNA SampleAPPENDIX H: Deionization of Formamide, Formaldehyde, and GlyoxalAPPENDIX I: Silanizing Centrifuge Tubes and GlasswareAPPENDIX J: Trypsinization Protocol for Anchorage-Dependent CellsAPPENDIX K: Isolation of High-Molecular-Weight DNA by Salting-OutAPPENDIX L: Electrophoresis: Principles, Parameters, and SafetyAPPENDIX M: Polyacrylamide Gel ElectrophoresisAPPENDIX N: Dot Blot Analysis (NEW)APPENDIX O: Centrifugation as a Mainstream Tool for the Molecular BiologistAPPENDIX P: Selected Suppliers of Equipment, Reagents, and ServicesAPPENDIX Q: Useful SI UnitsAPPENDIX R: Common AbbreviationsAPPENDIX S: Trade Citations
