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Full Description
Small GTPases play a key role in many aspects of contemporary cell biology: control of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the regulation of intracellular vesicular transport.This volume and its companions (Volumes 255, 256, 257, and the forthcoming 325) cover all biochemical and biological assays currently in use for analyzing the role of small GTPases in these aspects of cell biology at the molecular level.
Contents
Section I. Rab GTPases1: Structural Basis for Rab Function: An Overview2: Expression of Wild-Type and Mutant Green Fluorescent Protein-Rabl for Fluorescence Microscopy Analysis3: Fluorescence Methods for Monitoring Interactions of Rab Proteins with Nucleotides, Rab Escort Protein, and Geranylgeranyltransferase4: Prenylation of Rab Proteins in Vitro by Geranylgeranyltransferases5: Antibody and Oligonucleotide Probes to Distinguish Intracellular Expression and Localization Patterns of Rab GDP-Dissociation Inhibitor Isoforms6: Expression, Purification, and Biochemical Properties of Ypt/Rab GTPase-Activating Proteins of Gyp Family7: Purification and Properties of Rab3 GDP/GTP Exchange Protein8: Purification and Properties of Rab3 GTPase-Activating Protein9: Rabphilin-3: A Target Molecule for Rab3 Small G Proteins10: Doc2I+/- as Modulator of Ca2+-Dependent Exocytosis11: Purification and Properties of a GTPase-Activating Protein for Yeast Rab GTPases12: Purification and Characterization of Yeast Exocyst Complex13: Expression and Properties of Rab4 and Its Effector Rabaptin-4 in Endocytic Recycling14: Purification of EEA1 from Bovine Brain Cytosol Using Rab5 Affinity Chromatography and Activity Assays15: Expression, Purification, and Characterization of Rab5 Effector Complex, Rabaptin-5/Rabex-516: Measurement of Rab5 Protein Kinase B/akt and Regulation of Ras-Activated Endocytosis17: Expression, Purification, and Biochemical Properties of Rabkinesin-6 Domains and Their Interactions with Rab6A18: Expression and Functional Analyses of Rab8 and Rablla in Exocytic Transport from trans-Golgi Network19: Expression and Properties of Rab7 in Endosome Function20: Expression, Purification, and Properties of Rab8 Function in Actin Cortical Skeleton Organization and Polarized Transport21: Properties of Rab13 Interaction with Rod cGMP Phosphodiesterase I Subunit22: Subcellular Localization of Rab17 by Cryo-Immunogold Electron Microscopy in Epithelial Cells Grown on Polycarbonate Filters23: Expression and Properties of Rab25 in Polarized Madin-Darby Canine Kidney Cells24: Purification of TRAPP from Saccharomyces cerevisiae and Identification of Its Mammalian CounterpartSection II. ADP-Ribosylation Factor (ARF) GTPases25: Structural and Functional Organization of ADP-Ribosylation Factor (ARF) Proteins26: Expression and Properties of ADP-Ribosylation Factor (ARF6) in Endocytic Pathways27: Expression and Analysis of ARNO and ARNO Mutants and Their Effects on ADP-Ribosylation Factor (ARF)-Mediated Actin Cytoskeletal Rearrangements28: Expression, Purification, and Measurements of Activity of ARNO1, a Guanine Nucleotide Exchange Factor for ADP-Ribosylation Factor 1 (ARF1)29: Expression, Purification, and Biochemical Properties of EFA6, a Sec7 Domain-Containing Guanine Exchange Factor for ADP-Ribosylation Factor 6 (ARF6)30: Isolation and Properties of GRP1, an ADP-Ribosylation Factor (ARF)-Guanine Nucleotide Exchange Protein Regulated by Phosphatidylinositol 3, 4, 5-Trisphosphate31: Functional Analysis of ADP-Ribosylation Factor (ARF) Guanine Nucleotide Exchange Factors Gea1p and Gea2p in Yeast32: Isolation, Cloning, and Characterization of Brefeldin A-Inhibited Guanine Nucleotide-Exchange Protein for ADP-Ribosylation Factor33: Expression, Purification, and Properties of ADP-Ribosylation Factor (ARF) GTPase Activating Protein-134: Expression, Analysis, and Properties of Yeast ADP-Ribosylation Factor (ARF) GTPase Activating Proteins (GAPs) Gcs1 and Glo335: Purification and Properties of ARD1, An ADP-Ribosylation Factor (ARF)-Related Protein with GTPase-Activating Domain36: Purification and Characterization of GIT Family of ADP-Ribosylation Factor (ARF) GTPase-Activating Proteins37: Assay and Purification of Phosphoinositide-Dependent ADP-Ribosylation Factor (ARF) GTPase Activating Proteins38: Biological Properties and Measurement of Phospholipase D Activation by ADP-Ribosylation Factor (ARF)39: Use of Aminoglycoside Antibiotics and Related Compounds to Study ADP-Ribosylation Factor (ARF) /Coatomer Function in Golgi Traffic40: Adaptor Protein 1-Dependent Clathrin Coat Assembly on Synthetic Liposomes and Golgi Membranes41: Receptor-Dependent Formation of COPI-Coated Vesicles from Chemically Defined Donor Liposomes42: ADP-Ribosylation Factor (ARF) as Regulator of Spectrin Assembly at Golgi Complex43: Purification, Properties, and Analysis of yARL344: Preparation and Assay of Recombinant ADP-Ribosylation Factor-Like Protein-1 (ARL1)Section III. Sar GTPases45: Purification and Properties of Rat Liver Sec23aEURO"Sec24 Complex46: Purification of Functional Sec13paEURO"Sec31p Complex, A Subunit of COPII CoatSection IV. Dynamin GTPases47: Expression, Purification, and Functional Assays for Self-Association of Dynamin-148: Analysis of Phosphoinositide Binding by Pleckstrin Homology Domain from Dynamin49: Mapping Dynamin Interdomain Interactions with Yeast Two-Hybrid and Glutathione S-Transferase Pulldown Experiments50: Interactions of Dynamin and Amphiphysin with Liposomes51: Activation of Dynamin GTPase Activity by Phosphoinositides and SH3 Domain-Containing ProteinsSection V. Septin GTPases52: Expression and Analysis of Properties of Septin CDCrel-1 in ExocytosisAuthor IndexSubject Index
