Full Description
A comprehensive and accessible survey of the best current accomplishments of GMO research in all their complexity and ramifications. The authors introduce the fundamentals of biotechnology as a scientific discipline, show how GMO research is conducted today, discuss the problems that have arisen from genetic technology and the tools needed to resolve them, and describes how GMO-derived technology may impact our lives in the future. On the technical side, the authors examine a wide range of current technologies employed for constructing GMOs, and describe approaches to novel research, appropriate protocols, and the process of constructing and screening a GMO. The discussion of plant and animal cells covers new strategies employed and the large-scale expression and purification of recombinant products in cultured cells. Social political, and legal issues are also discussed.
Contents
I. Introduction.- 1 · Introduction.- II. Microbial GMOs.- 2 · Genetically Modified Microorganisms: Development and Applications.- 3 · Producing Proteins Derived From Genetically Modified Organisms for Toxicology and Environmental Fate Assessment of Biopesticides.- 4 · Genetically Modified Microorganisms: Biosafety and Ethical Issues.- III. Mammalian GMOs.- 5 · Large-Scale Exogenous Protein Production in Higher Animal Cells.- 6 · Biosafety, Ethics, and Regulation of Transgenic Animals.- 7 · Transgenic Aquatic Animals.- IV. Plant GMOs.- 8 · Development of Genetically Modified Agronomic Crops.- 9 · Gene Silencing in Plants: Nature's Defense.- 10 · Value Creation and Capture With Transgenic Plants.- 11 · Biosafety Issues, Assessment, and Regulation of Genetically Modified Food Plants.- 12 · Conclusion and Future Directions.