Cell Cycle Checkpoint Control Protocols (Methods in Molecular Biology (Clifton, N.J.), V. 241.)

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Cell Cycle Checkpoint Control Protocols (Methods in Molecular Biology (Clifton, N.J.), V. 241.)

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  • 製本 Hardcover:ハードカバー版/ページ数 372 p.
  • 言語 ENG
  • 商品コード 9781588291158
  • DDC分類 571.84

Full Description

The field of cell cycle regulation is based on the observation that the life cycle of a cell progresses through several distinct phases, G1, M, S, and G2, occurring in a well-defined temporal order. Details of the mechanisms involved are rapidly emerging and appear extraordinarily complex. Furthermore, not only is the order of the phases important, but in normal eukaryotic cells one phase will not begin unless the prior phase is completed successfully. Che- point control mechanisms are essentially surveillance systems that monitor the events in each phase, and assure that the cell does not progress prematurely to the next phase. If conditions are such that the cell is not ready to progress—for example, because of incomplete DNA replication in S or DNA damage that may interfere with chromosome segregation in M—a transient delay in cell cycle progression will occur. Once the inducing event is properly handled— for example, DNA replication is no longer blocked or damaged DNA is repaired—cell cycle progression continues. Checkpoint controls have recently been the focus of intense study by investigators interested in mechanisms that regulate the cell cycle. Furthermore, the relationship between checkpoint c- trol and carcinogenesis has additionally enhanced interest in these cell cycle regulatory pathways. It is clear that cancer cells often lack these checkpoints and exhibit genomic instability as a result. Moreover, several tumor suppressor genes participate in checkpoint control, and alterations in these genes are as- ciated with genomic instability as well as the development of cancer.

Contents

Part I. Induction and Detection of Changes in Cell Cycle Progression

Methods to Induce Cell Cycle Checkpoints
Howard B. Lieberman and Kevin M. Hopkins

Methods for Synchronizing Mammalian Cells
Michael H. Fox

Enrichment of Cells in Different Phases of the Cell Cycle by Centrifugal Elutriation
Tej K. Pandita

Analysis of the Mammalian Cell Cycle by Flow Cytometry
Haiying Hang and Michael H. Fox

Methods for Detecting Cells in S Phase
Wei-Hsin Sun and Melvin L. DePamphilis

Yeast Cell Synchronization
Audra Day, Colette Schneider, and Brandt L. Schneider

Analysis of the Budding Yeast Saccharomyces cerevisiae Cell Cycle by Morphological Criteria and Flow Cytometry
Hong Zhang and Wolfram Siede

Analysis of the Fission Yeast Schizosaccharomyces pombe Cell Cycle
Eliana B. Gómez and Susan L. Forsburg

Part II. Analysis of Genes Involved in Checkpoint Control

Strategies to Isolate Evolutionarily Conserved Cell Cycle Regulatory Genes
Scott Davey

Microarray Approaches for Analysis of Cell Cycle Regulatory Genes
Sally A. Amundson and Albert J. Fornace, Jr.

Using the Yeast Genome-Wide Gene-Deletion Collection for Systematic Genetic Screens
Jian Zhang, Lisa Ottmers, and Brandt L. Schneider

Gene Targeting in Cultured Human Cells
Todd A. Waldman

Use of In Vivo Gap Repair for Isolation of Mutant Alleles of a Checkpoint Gene
Migdalisel Colòn and Nancy C. Walworth

In Vitro Mutagenesis to Define Functional Domains
Jian Qin, Zhe Peng, and Maureen V. McLeod

Use of Gene Overexpression to Assess Function in Cell Cycle Control
Erik K. Flemington and Antonio Rodriguez

Histone Acetylation/Deacetylation As a Regulator of Cell Cycle Gene Expression
Chenguang Wang, Maofu Fu, and Richard G. Pestell

Part III. Analysis ofProteins Involved in Checkpoint Control

Cataloging Proteins in Cell Cycle Control
Kazimierz O. Wrzeszczynski and Burkhard Rost

Multidimensional Proteomic Analysis of Proteolytic Pathways Involved in Cell Cycle Control
Michael W. Schmidt, Aruna Jain, and Dieter A. Wolf

Purification and Identification of Protein Complexes That Control the Cell Cycle
Matthew A. Burtelow, Vladimir N. Podust, and Larry M. Karnitz

Xenopus Cell-Free Extracts to Study DNA Damage Checkpoints
Vincenzo Costanzo and Jean Gautier

Protein-Protein Interactions
Graziella Pedrazzi and Igor Stagljar

Detection of Kinase and Phosphatase Activities
Sean M. Post and Eva Y.-H.P. Lee

Monitoring Changes in the Subcellular Location of Proteins in S. cerevisiae
Diego Rua, Teresa Holzen, Benjamin S. Glick, Stephen J. Kron, and Douglas K. Bishop

Part IV. Chromosomes and the Cell Cycle

Chromosomal Changes and Cell Cycle Checkpoints in Mammalian Cells
Charles R. Geard and Brian Ponnaiya

Detecting the Influence of Cell Cycle Regulatory Proteins on Human Telomeres
Tej K. Pandita

Monitoring Spindle Assembly and Disassembly in Yeast by Indirect Immunofluorescence
Rita K. Miller

Index