Bacteriophages : Methods and Protocols (Methods in Molecular Biology)

個数：
  • ポイントキャンペーン

Bacteriophages : Methods and Protocols (Methods in Molecular Biology)

  • 在庫がございません。海外の書籍取次会社を通じて出版社等からお取り寄せいたします。
    通常6～9週間ほどで発送の見込みですが、商品によってはさらに時間がかかることもございます。
    【重要ご説明事項】
    1. 納期遅延や、ご入手不能となる場合がございます。
    2. 複数冊ご注文の場合、分割発送となる場合がございます。
    3. 美品のご指定は承りかねます。
  • 【入荷遅延について】
    世界情勢の影響により、海外からお取り寄せとなる洋書・洋古書の入荷が、表示している標準的な納期よりも遅延する場合がございます。
    おそれいりますが、あらかじめご了承くださいますようお願い申し上げます。
  • ◆画像の表紙や帯等は実物とは異なる場合があります。
  • ◆ウェブストアでの洋書販売価格は、弊社店舗等での販売価格とは異なります。
    また、洋書販売価格は、ご注文確定時点での日本円価格となります。
    ご注文確定後に、同じ洋書の販売価格が変動しても、それは反映されません。
  • 製本 Hardcover:ハードカバー版／ページ数 415 p.
  • 言語 ENG
  • 商品コード 9781071635483

Full Description

This volume provides detailed protocols for the isolation, enumeration, characterization of diverse bacteriophages, including both small and jumbo bacteriophages, from soil, fecal, municipal wastewater, and from food niche samples. Chapters highlight the diversity of bacteriophages in different environments, quantifications using culture and molecular techniques, protocols for isolation, interaction of bacteriophage proteins with host cells, and how to use bacteriophages to transfer foreign genetic elements to bacterial strains. In addition to the above, chapters feature the application of bacteriophages/bacteriophage-derived products. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips (in the Notes section) on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Bacteriophages: Methods and Protocols aims to ensure successful results in further study of this vital field.

Contents

​Structural and genomic diversity of bacteriophages.- The diversity of bacteriophages in human gut.- Breaking the ice - A review of phages from polar ecosystems.- The diversity of bacteriophages in hot spring.- Isolation of bacteriophage from soil samples in a poorly equipped field lab in Kruger National Park.- Purification and up-concentration of bacteriophages and viruses from fecal samples.- Isolation of enterococcus phages from municipal wastewater samples using an enrichment step.- Phage DNA extraction, genome assembly, and genome closure.- Enumeration of bacteriophages by plaque assay.- Detection and quantification of phages in wastewater samples by culture and molecular methods.- Flow virometry: A fluorescence-based approach to enumerate bacteriophages in liquid samples.- A metagenomics approach to enumerate bacteriophages in a Food Niche.- Bioinformatic analysis of Staphylococcus phages: a key step for safe cocktail development.- Use of localized reconstruction to visualize the Shigella-bacteriophage Sf6 tail apparatus.- Bacteriophage-host interactions and co-evolution.- Unraveling physical interactions of Clostridioides difficile with phage and phage-derived proteins using in vitro and whole-cell assays.- Phage Transduction of Staphylococcus aureus.- The Next Generation of Drug Delivery: Harnessing the Power of Bacteriophages.- Construction of nonnatural cysteine-crosslinked phage libraries.- Application of next generation sequencing in phage display.- The application of bacteriophage and photoacoustic flow cytometry in bacterial identification.- Propagation, purification and characterization of bacteriophages for phage.- Overcoming bacteriophage resistance in phage therapy.- .Bacteriophage virus-like particles: platforms for vaccine design.