Principles and Techniques of Practical Biochemistry (4 SUB)

Principles and Techniques of Practical Biochemistry (4 SUB)

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  • 製本 Hardcover:ハードカバー版/ページ数 586 p.
  • 言語 ENG
  • 商品コード 9780521417693
  • DDC分類 574.192

Table of Contents

Preface to the fifth edition                       xiii
List of contributors xvi
List of abbreviations xviii
General principles of biochemical
investigations
I. Simpkins
The nature of biochemistry 1 (3)
Bioenergetics 4 (17)
Methods for investigating metabolism 21 (7)
Practical considerations 28 (14)
In vivo models 42 (2)
In vitro models 44 (22)
Microscopy 66 (9)
Key terms 75 (1)
Calculations 76 (2)
Suggestions for further reading 78 (2)
Molecular biology and basic techniques 80 (58)
R. Rapley
Introduction 80 (1)
Components and primary structure of nucleic 80 (7)
acids
Genes and genome complexity 87 (3)
The nature of the genetic code 90 (1)
Cellular location of nucleic acids 90 (3)
The cellular functions of DNA 93 (10)
The manipulation of nucleic acids: basic 103 (2)
tools and techniques
Isolation and separation of nucleic acids 105 (5)
Restriction mapping of DNA fragments 110 (1)
Nucleic acid blotting methods 111 (2)
Gene probe derivation 113 (1)
Labelling DNA gene probe molecules 114 (2)
The polymerase chain reaction 116 (9)
Nucleotide sequencing of DNA 125 (6)
Bioinformatics and the Internet 131 (3)
Key terms 134 (1)
Calculations 135 (1)
Suggestions for further reading 136 (2)
Molecular cloning and gene analysis 138 (68)
R. Rapley
Introduction 138 (1)
Constructing gene libraries 138 (10)
Cloning vectors 148 (19)
Hybridisation and gene probes 167 (1)
Screening gene libraries 167 (4)
Applications of gene cloning 171 (7)
Expression of foreign genes 178 (6)
Analysing genes and gene expression 184 (12)
Analysing whole genomes 196 (6)
Molecular biotechnology and applications 202 (2)
Key terms 204 (1)
Suggestions for further reading 205 (1)
Immunochemical techniques 206 (57)
R. Thorpe
S. Thorpe
Introduction 206 (5)
Production of antibodies 211 (11)
Purification and fragmentation of 222 (7)
immunoglobulins
Immunoprecipitation 229 (5)
Labelling antibodies 234 (6)
Immunoblotting 240 (4)
Immunoassays 244 (10)
Immunohisto/cytochemistry 254 (6)
Affinity and avidity 260 (1)
Immunochemical use of surface plasmon 260 (1)
resonance
Key terms 261 (1)
Calculation 262 (1)
Suggestions for further reading 262 (1)
Centrifugation techniques 263 (49)
A. Griffiths
Introduction 263 (1)
Basic principles of sedimentation 264 (7)
Centrifuges and their uses 271 (5)
Design and care of preparative rotors 276 (8)
Sample containers 284 (1)
Separation methods in preparative 285 (5)
ultracentrifuges
Performing density gradient separations 290 (6)
Selection, efficiency and applications of 296 (4)
preparative rotors
Analysis of subcellular fractions 300 (2)
Some applications of the analytical 302 (3)
ultracentrifuge
Safety aspects in the use of centrifuges 305 (1)
Key terms 306 (1)
Calculations 306 (5)
Suggestions for further reading 311 (1)
Protein structure, purification and 312 (45)
characterisation
J. Walker
Ionic properties of amino acids and proteins 312 (4)
Protein structure 316 (2)
Protein purification 318 (20)
Protein structure determination 338 (15)
Key terms 353 (1)
Calculations 354 (2)
Suggestions for further reading 356 (1)
Biomolecular interactions: I Enzymes 357 (46)
K. Wilson
Receptor--ligand binding 357 (1)
Enzymes: characteristics and nomenclature 358 (1)
Enzyme steady-state kinetics 359 (19)
Enzyme assays 378 (7)
Substrate assays 385 (1)
Enzyme pre-steady-state kinetics 386 (3)
Enzyme active sites and catalytic mechanisms 389 (5)
Immobilised enzymes 394 (2)
Cellular control of metabolic activity 396 (4)
Key terms 400 (1)
Calculations 401 (1)
Suggestions for further reading 402 (1)
Biomolecular interactions: II Cell surface 403 (50)
receptors and transporters
K. Wilson
Cell surface receptor classification 403 (3)
Quantitative aspects of receptor-ligand 406 (11)
binding
Receptor structures 417 (3)
Mechanisms of signal transduction 420 (10)
Signal amplification 430 (2)
Key terms 432 (1)
Membrane transport processes 432 (1)
Physical diffusion 433 (2)
Facilitated transport 435 (3)
Active transport and ion channels 438 (7)
Receptor-mediated endocytosis 445 (4)
Key terms 449 (1)
Calculations 449 (2)
Suggestions for further reading 451 (2)
Spectroscopic techniques: I Atomic and 453 (45)
molecular electronic spectroscopy
D. B. Gordon
Introduction 453 (3)
γ-Ray spectroscopy and γ-ray 456 (2)
resonance spectroscopy
X-ray spectroscopy 458 (1)
Ultraviolet and visible light spectroscopy 459 (12)
Spectrofluorimetry 471 (8)
Circular dichroism spectroscopy 479 (3)
Turbidimetry and nephelometry 482 (1)
Luminometry 483 (2)
Atomic spectroscopy 485 (5)
Lasers 490 (1)
Key terms 491 (1)
Calculations 492 (5)
Suggestions for further reading 497 (1)
Spectroscopic techniques: II Vibrational 498 (29)
spectroscopy and electron and nuclear spin
orientation in magnetic fields
D. B. Gordon
Introduction 498 (1)
Infrared and Raman spectroscopy 498 (3)
Electron spin resonance spectroscopy 501 (7)
Nuclear magnetic resonance spectroscopy 508 (17)
Key terms 525 (1)
Suggestions for further reading 526 (1)
Mass spectrometric techniques 527 (53)
D. B. Gordon
Introduction 527 (1)
The mass spectrometer 527 (2)
Electron impact ionisation 529 (9)
Chemical ionisation 538 (1)
Field ionisation 539 (1)
Ion desorption methods 539 (12)
Ion evaporation methods 551 (4)
Analysers 555 (9)
Detectors 564 (2)
Tandem mass spectrometry 566 (7)
Key terms 573 (1)
Calculations 573 (6)
Suggestions for further reading 579 (1)
Electrophoretic techniques 580 (39)
J. M. Walker
General principles 580 (4)
Support media 584 (4)
Electrophoresis of proteins 588 (19)
Electrophoresis of nucleic acids 607 (5)
Capillary electrophoresis 612 (5)
Key terms 617 (1)
Calculation 618 (1)
Suggestions for further reading 618 (1)
Chromatographic techniques 619 (68)
K. Wilson
Introduction 619 (4)
Chromatography theory and practice 623 (8)
Low pressure column chromatography 631 (6)
High performance liquid chromatography 637 (10)
Adsorption chromatography 647 (2)
Partition chromatography 649 (7)
Ion-exchange chromatography 656 (5)
Molecular exclusion (permeation) 661 (4)
chromatography
Affinity chromatography 665 (7)
Gas--liquid chromatography 672 (6)
Thin-layer (planar) chromatography 678 (3)
Selection of a chromatographic system 681 (1)
Key terms 682 (1)
Calculations 683 (3)
Suggestions for further reading 686 (1)
Radioisotope techniques 687 (42)
R. J. Slater
The nature of radioactivity 687 (6)
Detection and measurement of radioactivity 693 (20)
Other practical aspects of counting 713 (4)
radioactivity and analysis of data
Inherent advantages and restrictions of 717 (1)
radiotracer experiments
Safety aspects 718 (3)
Applications of radioisotopes in the 721 (5)
biological sciences
Key terms 726 (1)
Calculations 726 (2)
Suggestions for further reading 728 (1)
Electrochemical techniques 729 (42)
P. K. Robinson
Introduction 729 (5)
Principles of electrochemical techniques 734 (8)
Redox reactions 742 (3)
The pH electrode 745 (3)
Ion-selective and gas-sensing electrodes 748 (2)
The Clark oxygen electrode 750 (7)
Electrochemical detectors for HPLC 757 (3)
Biosensors 760 (8)
Key terms 768 (1)
Calculations 768 (2)
Suggestions for further reading 770 (1)
Index 771