Description
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers recent research and methods development for changing the DNA sequence within the genomes of cells and organisms. Focusing on enzymes that generate double-strand breaks in DNA, the chapters describe use of molecular tools to introduce or delete genetic information at specific sites in the genomes of animal, plant and bacterial cells.
- Continues the legacy of this premier serial with quality chapters authored by leaders in the field
- Covers research methods in biomineralization science
- Contains sections on such topics as genome editing, genome engineering, CRISPR, Cas9, TALEN and zinc finger nuclease
Table of Contents
1. In Vitro Enzymology of Cas9
Carolin Anders and Martin Jinek
2. Targeted Genome Editing in Human Cells Using Crispr-Cas Nucleases and Truncated Guide Rnas
Yanfang Fu, Deepak Reyon and J. Keith Joung
3. Determining the Specificities of Talens, Cas9, and Other Genome Editing Enzymes
Vikram Pattanayak , John P. Guilinger,and David R. Liu
4. Genome Engineering with Custom Recombinases
Thomas Gaj, and Carlos F. Barbas
5. Genome Engineering in Human Cells
Minjung Song, Young-Hoon Kim, Hyongbum Kim1 and Jin-Soo Kim
6. Genome Editing in Human Stem Cells
Susan M. Byrne, Prashant Mali, and George M. Church
7. Tagging Endogenous Loci for Live-Cell Fluorescence Imaging and Molecule Counting Using ZFNs, Talens and Cas9
Dambournet D., Hong SH. , Grassart A and Drubin DG
8. Genome Editing Using Cas9 Nickases
Alexandro E. Trevino and Feng Zhang
9. Assaying Break and Nick-Induced Homologous Recombination in Mammalian Cells Using the DR-GFP Reporter and Cas9 Nucleases
Lianne E.M. Vriend , Maria Jasin and Przemek M. Krawczyk
10. Adapting CRISPR/Cas9 for Functional Genomics Screens
Abba Malina , Alexandra Katigbak, Regina Cencic, Rayelle Itoua Maïga, Francis Robert, Hisashi Miura and Jerry Pelletier
11. The iCRISPR Platform for Rapid Genome Editing in Human Pluripotent Stem Cells
Zengrong Zhu , Federico González and Danwei Huangfu
12. Creating Cancer Translocations in Human Cells Using Cas9 DSBs and nCas9 Paired Nicks
Benjamin Renouf, Marion Piganeau, Hind Ghezraoui, Maria Jasin and Erika Brunet
13. Genome Editing for Human Gene Therapy
Torsten B. Meissner, Pankaj K. Mandal, Leonardo M. R. Ferreira, Derrick J. Rossi and Chad A. Cowa
14. Generation of Site-Specific Mutations in the Rat Genome Via CRISPR-Cas9
Yuting Guan, Yanjiao Shao, Dali Li and Mingyao Liu
15. CRISPR-Cas9-Based Genome Editing in Mice by Single Plasmid Injection
Yoshitaka Fujihara and Masahito Ikawa
16. Imaging Genomic Elements in Living Cells using CRISPR/Cas9
Baohui Chen and Bo Huang
17. Cas9-Based Genome Editing in Xenopus Tropicalis
Takuya Nakayama, Ira L. Blitz, Margaret B. Fish, Akinleye O. Odeleye, Sumanth Manohar, Ken W.Y. Cho and Robert M. Grainger
18. Cas9-Based Genome Editing in Zebrafish
Andrew P.W. Gonzales, and Jing-Ruey Joanna Yeh
19. Cas9-Based Genome Editing in Drosophila
Benjamin E. Housden , Shuailiang Lin and Norbert Perrimon
20. Transgene Free Genome Editing by Germline Injection of CRISPR Cas RNA
Hillel T Schwartz and Paul W Sternberg
21. Cas9-Based Genome Editing in Arabidopsis and Tobacco
Jian-Feng Li, Dandan Zhang, and Jen Sheen
22. Multiplex Engineering of Industrial Yeast Genomes Using CRISPRm
Owen W. Ryan1 and Jamie H. D. Cate
23. Protein Engineering of Cas9 for Enhanced Function
Benjamin L. Oakes, Dana C. Nadler, and David F. Savage
