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Full Description
The latest and most widely employed approaches for evaluating essential enzymes involved in carbon, nitrogen, protein, and lipid metabolism, along with the estimation of various plant pigments and micro and macro elements, are discussed in this book. In addition, the quantification of plant hormones such as IAA, ABA, GA, and Ethylene is covered. The book also explores techniques for the estimation of DNA and RNA, as well as Slab Gel Electrophoresis and Western Blot analysis of plant proteins. The study of plant biomass and plant-water relationships, as well as methods for measuring photosynthesis and respiration, are also included. Furthermore, the book provides details on the techniques for preparing a common buffer, the working principles and operation techniques of various analytical equipment, including the Infra-Red Gas Analyzer (IRGA), Gas Liquid Chromatograph (GLC), Psychrometer, Pressure bomb/pressure chamber, flame photometer, atomic absorption spectrophotometer, Leaf Area Meter, and Oxygen electrode. This book is an essential resource for students in botany, plant physiology, biochemistry, horticulture, agronomy, and other related disciplines, as well as other research workers.
Contents
1. Plant pigments.
2. Biomass.
3. Growth analysis.
4. Water relations.
5. Carbohydrate metabolism.
6. Nitrogen and amino acid metabolism.
7. Protein and protein fractionation.
8. Isolation and estimation of nucleic acids.
9. Lipid and lipid metabolism.
10. Photosynthesis.
11. Respiration.
12. The antioxidative enzymes.
13. Ripening-associated enzymes.
14. Plant growth regulators.
15. Ascorbic acid.
16. Phenols.
17. Macro and micro elements.
18. Isolation of cell organelles.
19. Common buffers.
