Full Description
Microsatellites or simple sequence repeats (SSRs) have become the markers of choice for a variety of molecular studies because of their versatility, operational flexibility, and lower cost than other marker systems. Microsatellites: Methods and Protocols brings together experts in the field to cover this significant area of research. Broken in to four convenient parts, this volume delves into classical and modern methods for the discovery and development of microsatellite markers, descriptions of amplification and visualization of SSRs, automated capillary sequencers that are widely used for fragment analysis, as well as a variety of methods for the analysis of data obtained by the use of microsatellites. Written for the highly successful Methods in Molecular Biology™ series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and invaluable, Microsatellites: Methods and Protocols aims at researchers that need detailed protocols for incorporating microsatellite markers into their projects and expert scientists looking to expand their knowledge of SSRs discovery, use, and analysis.
Contents
Microsatellites: Evolution and Contribution.- Screening of Genomic Libraries.- PCR-Based Identification of Microsatellite Arrays (PIMA).- Fast Isolation by AFLP of Sequences Containing Repeats (FIASCO).- Microsatellite DNA Capture from Enriched Libraries.- Next-Generation Sequencing for High-Throughput Molecular Ecology: A Step-by-Step Protocol for Targeted Multilocus Genotyping by Pyrosequencing.- Optimizing Selection of Microsatellite Loci from 454 Pyrosequencing Via Post-Sequencing Bioinformatic Analyses.- Identification of DNA-Microsatellite Markers for the Characterization of Somatic Embryos in Quercus suber.- Simple Sequence Repeats (SSR) Amplification.- Microsatellite Amplification in Plants: Optimization Procedure of Major PCR Components.- Development of a Multiplex PCR Assay for Characterization of Embryonic Stem Cells.- Agarose Gel Electrophoresis and Polyacrylamide Gel Electrophoresis for Visualization of Single Sequence Repeats.- Microsatellite Fragment Analysis Using the ABI Prism® 377 DNA Sequencer.- Robust and Inexpensive SSR Markers Analyses Using LI-COR DNA Analyzer.- The Use of the MegaBACE for Sequencing and Genotype Analysis.- Analyzing Microsatellites Using the QIAxcel System.- Microsatellite Analysis of Malaria Parasites.- Informativeness of Microsatellite Markers.- Microsatellite Data Analysis for Population Genetics.- Molecular Mapping and Breeding with Microsatellite Markers.
