Full Description
A cutting-edge collection of readily reproducible techniques for the isolation, culture, and study of activation and signaling in human mast cells. These methods take advantage of the latest advances in molecular biology, technology, and information science. They include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis.
Contents
Overview of Mast Cell Biology.- Paul Ehrlich''s ";Mastzellen"— From Aniline Dyes to DNA Chip Arrays.- The Human Mast Cell.- Mast Cells in Allergy and Autoimmunity.- Identification of Mast Cells in Culture and in Tissue.- Analysis of MCT and MCTC Mast Cells in Tissue.- Mast Cell Ultrastructure and Staining in Tissue.- Expression of Cell Surface Antigens on Mast Cells.- Identification of Mast Cells in the Cellular Response to Myocardial Infarction.- Development of Mast Cells In Vitro.- Growth of Human Mast Cells From Bone Marrow and Peripheral Blood-Derived CD34+ Pluripotent Progenitor Cells.- Culture of Human Mast Cells From Hemopoietic Progenitors.- Isolation, Culture, and Characterization of Intestinal Mast Cells.- Mast Cell Signaling and Gene Expression.- Activation of Nuclear Factor-?B.- Analysis of Mitogen-Activated Protein Kinase Activation.- Microarray and Gene-Clustering Analysis.- Techniques to Study Fc?RI Signaling.- Mast Cell Expression and Release of Inflammatory Mediators of Inflammatory Mediators.- Human Mast Cell Proteases.- Mast Cell Histamine and Cytokine Assays.- Measurement of Mast Cell Cytokine Release by Multiplex Assay.- Assays for Histamine-Releasing Factors.- Assays for Nitric Oxide Expression.- Immunohistological Detection of Growth Factors and Cytokines in Tissue Mast Cells.- Mast Cell Interactions With Other Cell Types.- Endothelial Cell Activation by Mast Cell Mediators.- Co-Culture of Mast Cells With Fibroblasts.- Detection of ? Class Switching and IgE Synthesis in Human B Cells.- Novel Aspects of Mast Cell Activation and Regulation.- Gene Silencing Using Small Interference RNA in Mast Cells.- Mast Cell Activation by Lipoproteins.- Mast Cell Activation by Stress.- Roles of Mast Cells in Host Defense.- In Vivo Models for StudyingMast Cell-Dependent Responses to Bacterial Infection.- Bacterial Activation of Mast Cells.- Activation of Mast Cells by Streptolysin O and Lipopolysaccharide.- Analysis of Programmed Cell Death in Mast Cells.- Mast Cell Apoptosis.
