Bone Research Protocols

Bone Research Protocols

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  • 製本 Hardcover:ハードカバー版/ページ数 500 p.
  • 言語 ENG
  • 商品コード 9781588290441
  • DDC分類 612.75072

基本説明

The book's focus is on laboratory, rather than clinical, protocols and on those that are specific to bone and bone tissue only, such as the special considerations that apply to isolation of DNA and RNA from bone.

Table of Contents

Preface                                            v
Contributors ix
Part I Culture of Osteoblasts and Osteocytes
Human Osteoblast Culture 3 (16)
James A. Gallagher
Osteoblast Isolation from Murine Calvariae 19 (10)
and Long Bones
Astrid Bakker
Jenneke Klein-Nulend
Mineralizing Fibroblast-Colony-Forming 29 (12)
Assays
Andrew Scutt
Lynsey Reading
Nanette Scutt
Karen Still
Osteocyte Isolation and Culture 41 (12)
Peter J. Nijweide
Arie van der Plas
Marcel J. Alblas
Jenneke Klein-Nulend
Part II Culture of Osteoclasts
Isolated Osteoclast Cultures 53 (12)
Astrid Hoebertz
Timothy R. Arnett
Primary Isolation and Culture of Chicken 65 (24)
Osteoclasts
Patricia Collin-Osdoby
Fred Anderson
Philip Osdoby
Isolation and Purification of Rabbit 89 (12)
Osteoclasts
Fraser P. Coxon
Julie C. Frith
Helena L. Benford
Michael J. Rogers
Generating Human Osteoclasts from 101(12)
Peripheral Blood
Afsie Sabokbar
Nicholas A. Athanasou
Generating Human Osteoclasts from Bone 113(16)
Marrow and Peripheral Blood
Adrienne M. Flanagan
Helen M. Massey
Generating Murine Osteoclasts from Bone 129(16)
Marrow
Naoyuki Takahashi
Nobuyuki Udagawa
Sakae Tanaka
Tatsuo Suda
Osteoclast Formation in the Mouse Coculture 145(8)
Assay
Robert J. van 't Hof
RANKL-Mediated Osteoclast Formation from 153(16)
Murine RAW 264.7 Cells
Patricia Collin-Osdoby
Xuefeng Yu
Hong Zheng
Philip Osdoby
Part III Bone Organ Culture
Analysis of Osteoclast Function in Mouse 169(14)
Calvarial Cultures
Michael J. Marshall
Marit N. Rowlands
Assessing Bone Formation Using Mouse 183(18)
Calvarial Organ Culture
I. Ross Garrett
Part IV Histological and Ultrastructural
Techniques
In Situ Hybridization and In Situ Reverse 201(24)
Transcription Polymerase Chain Reaction in
Human Bone Sections
Andrew P. Mee
Judith A. Hoyland
Techniques for the Study of Apoptosis in 225(12)
Bone
Brendon S. Noble
Hazel Y. Stevens
Protein Localization in Wax-Embedded and 237(12)
Frozen Sections of Bone Using
Immunohistochemistry
Sharyn Bord
Detection of Noncollagenous Bone Proteins 249(10)
in Methylmethacrylate-Embedded Human Bone
Sections
Johannes P. T. M. van Leeuwen
Pieter Derkx
Fluorescence Imaging of Bone-Resorbing 259(24)
Osteoclasts by Confocal Microscopy
Stephen A. Nesbitt
Michael A. Horton
Bone Histomorphometry 283(16)
Shobna Vedi
Juliet Compston
Transmission Electron Microscopy of Bone 299(12)
Vincent Everts
Anneke Niehof
Wouter Beertsen
Scanning Electron Microscopy of Bone 311(12)
Deborah Marshall
Miep H. Helfrich
Richard M. Aspden
Part V Imaging Techniques
Bone Measurements by Peripheral 323(22)
Quantitative Computed Tomography in Rodents
Jurg A. Gasser
Part VI In Vivo Techniques
Studies of Local Bone Remodeling: The 345(8)
Calvarial Injection Assay
Robert J. van 't Hof
Claire E. Clarkin
Kenneth J. Armour
Inflammation-Induced Osteoporosis: The IMO 353(8)
Model
Kenneth J. Armour
Katharine E. Armour
Ovariectomy and Estrogen Replacement in 361(8)
Rodents
Jade W. M. Chow
Part VII Mechanical Loading Techniques
Mechanical Testing of Bone Ex Vivo 369(12)
Richard M. Aspden
Bone Cell Responses to Fluid Flow 381(18)
Hazel Y. Stevens
John A. Frangos
Methods for Analyzing Bone Cell Responses 399(26)
to Mechanical Loading Using In Vitro
Monolayer and Organ Culture Models
Andrew A. Pitsillides
Victoria Das-Gupta
Dominic Simon
Simon C. F. Rawlinson
Part VIII Molecular Analysis
Extraction of Nucleic Acids from Bone 425(8)
Tracy L. Stewart
Val Mann
Analysis of Gene Expression in Bone by 433(8)
Quantitative RT-PCR
Deborah Ireland
Index 441