Full Description
This second edition volume expands on the previous edition with descriptions of recent developments in the field. The chapters in this book cover topics such as monoclonal antibodies for the treatment of melanoma; production and purification of human monoclonal antibodies; humanization and optimization of monoclonal antibodies; rapid chimerization of monoclonal antibodies; epitope mapping via phage display from single gene libraries; recombinant antibodies made by combining phage and yeast display selections; production of stabilized antibody fragments in the E. coli bacterial cytoplasm and transfected mammalian cells; and analysis of CAR T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Uniqueand thorough, Human Monoclonal Antibodies: Methods and Protocols, Second Edition is a valuable tool for novice and expert researchers interested in learning more about this evolving field.
Contents
Human Monocolonal Antibodies: The Benefits of Humanization.- Cancer Immunotherapy: The Dawn of Antibody Cocktails.- IgM Natural Autoantibodies in Physiology and the Treatment of Disease.- Monoclonal Antibodies for the Treatment of Melanoma: Present and Future Strategies.- An Efficient Method to Generate Monoclonal Antibodies from Human B Cells.- Isolation of Antigen-Specific, antibody-Secreting Cells using a Chip-Based Immunospot Array.- Purification of Human Monoclonal antibodies and Their Fragments.- One-Tube Multicolor Flow Cytometry Assay (OTMA) for Comprehensive Immunophenotyping of Peripheral Blood.- Humanization and Simultaneous Optimization of Monoclonal Antibody.- Antibody Fragments Humanization: Beginning with the End in Mind.- Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice.- Refining the Quality of Monoclonal Antibodies: Grafting Unique Peptide-Binding Site in the Fab Framework.- BasicProcedures for Detection and Cytotoxicity of Chimeric Antigen Receptors.- Rapid Chimerization of Antibodies.- Phage Display Technology for Human Monoclonal Antibodies.- Recombinant Antibody Selections by Combining Phage and Yeast Display.- Epitope Mapping via Phage Display from Single Gene Libraries.- A High-Throughput Magnetic Nanoparticle Based Semi-Automated Antibody Phage Display Biopanning.- A Binding Potency Assay for Pritumumab and Ecto-Domain Vimentin.- A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors.- A Cell-Based Reporter Assay Measuring the Activation of Fc Gamma Receptors Induced by Therapeutic Monoclonal Antibodies.- "BIClonals": Production of Bispecific Antibodies in IgG Format in Transiently Transfected Mammalian Cells.- Production of Stabilized Antibody Fragments in the E. coli Bacterial Cytoplasm and in Transiently-Transfected Mammalian Cells.
