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Full Description
Estimation of the metabolite complement of plant material involves a wide range of techniques and technologies and that breadth continues to increase. Metabolomics research typically involves multiple sites for material preparation and analysis and most investigations are "high throughput", meaning that chemical analysis of sample sets are inevitably carried out over an extended period of time. In, Plant Metabolomics: Methods and Protocols expert researchers in the field detail many of the stages which are now commonly used to study plant metabolomics workflow. Stages of this workflow, up to and including the statistical analysis, accurate and detailed collection of meta-data are also essential for good process management, to satisfy reporting requirements and to ensure wider interpretability and reuse results.Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Through and intuitive Plant Metabolomics: Methods and Protocols, seeks to aid scientists in the further study of the methods for all the stages of the plant metabolomics workflow.
Contents
Practical Applications of Metabolomics in Plant Biology.-Aspects of Experimental Design for Plant Metabolomics Experiments and Guidelines for Growth of Plant Material.-Separating the Inseparable: The Metabolomic Analysis of Plant - Pathogen Interactions.-Precautions for Harvest, Sampling, Storage and Transport of Crop Plant Metabolomics Samples.-Tissue Preparation using Arabidopsis.-Solid Phase Micro-Extraction (SPME) GC - MS Analysis of Natural Volatile Components in Melon and Rice.-Profiling Primary Metabolites of Tomato Fruit with Gas Chromatography/Mass-Spectrometry.-High Performance Liquid Chromatography (HPLC)- Mass Spectrometry (MS) Analysis of Plant Metabolites in Brassicaceae.-UPLC-MS-based Metabolite Analysis in Tomato.-High Precision Measurement and Fragmentation Analysis for Metabolite Identification.-Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) for Plant Metabolite Profiling and Metabolite Identification.-Combined NMR and Flow Injection ESI-MS for Brassicaceae Metabolomics.-ICP-MS and LC-ICP-MS for Analysis of Trace Element Content and Speciation in the Cereal Grain.-The Use of Genomics and Metabolomics Methods to Quantify Fungal Endosymbionts and Alkaloids in Grasses.-Data (pre-)processing of Nominal and Accurate Mass LC-MS or GC-MS Data using MetAlign.-TagFinder-Preprocessing Software for the Fingerprinting and the Profiling of Gas Chromatography-Mass Spectrometry(GC-MS) Based Metabolome Analyses.-Chemical Identification Strategies using Liquid Chromatography-photo Diode Array-solid PhaseExtraction-nuclear Magnetic Resonance / Mass Spectrometry.-A Strategy for Selecting Data Mining Techniques in Metabolomics.
