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Full Description
This volume explores the latest techniques and methods used for performing up-to-date glycosylation research. The chapters in this book are organized into four parts. Part One looks at the latest analytical and bioinformatics technologies that enable the characterization of glycosylation complexity. Part Two details the importance of synthetic chemistry and glycoengineering in the fields of bioprocessing and biotherapeutic development. Part Three discusses systems biology and computational technologies used by scientists to analyze glycosylation events in the cell. Part Four focuses on how cellular glycosylation biomarkers can be identified and used to characterize human clinical datasets. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Glycosylation: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this exciting and developing field.
Contents
Glycoinformatics Tools for Comprehensive Characterization of Glycans Enzymatically Released from Proteins.- GlycoStore: A Platform for H/UPLC and Capillary Electrophoresis Glycan Data.- An Interactive View of Glycosylation.- Characterization and Analysis of Food-Sourced Carbohydrates.- Comparative Glycomics Analysis of Mass Spectrometry Data.- Cell Free Remodeling of Glycosylation of Antibodies.- Rapid Antibody Glycoengineering in CHO Cells via RNA Interference and CGE-LIF N-Glycomics.- In Silico Analysis of Therapeutic Antibody Aggregation and the Influence of Glycosylation.- Recent Advances Towards Engineering Glycoproteins using Modified Yeast Display Platforms.- Computational Modeling of Glycan Processing in the Golgi for Investigating Changes in the Arrangements of Biosynthetic Enzymes.- O-Glycologue: A Formal-Language-Based Generator of O-Glycosylation Networks.- Synthetic Strategies for FRET-Enabled Carbohydrate ActiveEnzyme Probes.- N-Glycan Characterization by Liquid Chromatography Coupled with Fluorimetry and Mass Spectrometry.- Lectin Histochemistry for Tissues and Cells, and Dual Lectin and Antibody Co-Localization.- Lectin Affinity Chromatography for the Discovery of Novel Cancer Glycobiomarkers: A Case Study with PSA Glycoforms and Prostate Cancer.- Metabolic Labelling of Primary Neurons using Carbohydrate Click-Chemistry.- Monitoring the Sialome on Human Immune Cells.
