植物の環状RNA：研究法・プロトコル Plant Circular RNAs : Methods and Protocols (Methods in Molecular Biology)

植物の環状RNA：研究法・プロトコル<br>Plant Circular RNAs : Methods and Protocols (Methods in Molecular Biology)

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植物の環状RNA：研究法・プロトコル
Plant Circular RNAs : Methods and Protocols (Methods in Molecular Biology)

Vaschetto, Luis María (EDT)

ウェブストア価格 ¥43,364（本体¥39,422）
Springer-Verlag New York Inc.（2021/07発売）
外貨定価 US$ 199.99
ポイント 394pt

オンデマンド(OD/POD)版です。キャンセルは承れません。

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製本 Hardcover:ハードカバー版／ページ数 196 p.
言語 ENG
商品コード 9781071616444
DDC分類 580

Full Description

This volume presents readers with up-to-date protocols, bioinformatics toolkits, and reference material for understanding circRNAs in plants. The chapters in this book summarize the concepts and techniques for prediction/identification, validation, and analysis of plant circRNAs and their regulatory targets. Some of the topics covered are procedures for circRNA identification and characterization; strategies for circRNA generation; next generation sequencing (NGS) technologies and circRNAs' research; and bioinformatics tools and pipelines for the analysis of circRNA-miRNA-mRNA regulatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Circular RNAs: Methods and Protocols is an important reference for any researcher looking to explore and apply the latest developments made in this field.

Identification and Characterization of Plant Circular RNAs.- Experimental Strategies for Studying the Function of Plant circRNAs.- Generation of Transgenic Rice Expressing circRNA and its Functional Characterization.- Identification of Circular RNAs by Multiple Displacement Amplification and their Involvement in Plant Development.- Identification of Intronic Lariat-Derived Circular RNAs in Arabidopsis by RNA Deep Sequencing.- Identification and Functional Characterization of Viroid Circular RNAs.- Circular RNA Databases.- NGS Methodologies and Computational Algorithms for the Prediction and Analysis of Plant Circular RNAs.- Computational Analysis of Transposable Elements and circRNAs in Plants.- Constructing circRNA-miRNA-mRNA Regulatory Networks by using GreenCircRNA Database.- Methods for Predicting circRNA-miRNA-mRNA Regulatory Networks: GreenCircRNA and PlantCircNet Databases as Study Cases.