Embryonic Stem Cells : Methods and Protocols (Methods in Molecular Biology)

Embryonic Stem Cells : Methods and Protocols (Methods in Molecular Biology)

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  • 製本 Hardcover:ハードカバー版/ページ数 512 p.
  • 言語 ENG,ENG
  • 商品コード 9780896038813
  • DDC分類 612.0181

Full Description

Kursad Turksen and a panel of international experts describe their most productive methods for using embryonic stem (ES) cells as in vitro developmental models for many cell and tissue types. Set out in step-by-step detail, these protocols range widely from ES cell isolation, maintenance, and modulation of gene expression, to cutting-edge techniques that use cDNA arrays in gene expression analysis and phage display libraries, the generation of antibodies against very rare antigens, and the identification of and characterization of protein and protein interactions. Embryonic Stem Cells: Methods and Protocols will prove an invaluable resource not only for those interested generally in cell and developmental biology, but also for those actively using, or planning to use ES cells, to study fate choices and specific lineages.

Table of Contents

Preface                                            v
Contributors xi
Color Plates xv
Methods for the Isolation and Maintenance of 1 (16)
Murine Embryonic Stem Cells
Marsha L. Roach
John D. McNeish
The Use of a Chemically Defined Media for the 17 (10)
Analyses of Early Development in ES Cells and
Mouse Embryos
Gabriele Proetzel
Michael V. Wiles
Analysis of the Cell Cycle in Mouse Embryonic 27 (8)
Stem Cells
Pierre Savatier
Helene Lapillonne
Ludmila Jirmanova
Luigi Vitelli
Jacques Samarut
Murine Embryonic Stem Cells as a Model for 35 (10)
Stress Proteins and Apoptosis During
Andre-Patrick Arrigo
Patrick Mehlen
Effects of Altered Gene Expression on ES Cell 45 (10)
Young Fan
J. Richard Chaillet
Hypoxic Gene Regulation in Differentiating ES 55 (8)
David M. Adelman
M. Celeste Simon
Regulation of Gap Junction Protein (Connexin) 63 (8)
Genes and Function in Differentiating ES Cells
Masahito Oyamada
Yumiko Oyamada
Tomoyuki Kaneko
Tetsuro Takamatsu
Embryonic Stem Cell Differentiation as a 71 (12)
Model to Study Hematopoietic and Endothelial
Cell Development
Stuart T. Fraser
Minetaro Ogawa
Satomi Nisikawa
Shin-Ichi Nishikawa
Analysis of Bcr-Abl Function Using an in 83 (14)
Vitro Embryonic Stem Cell Differentiation
Takumi Era
Stephane Wong
Owen N. Witte
Embryonic Stem Cells as a Model for Studying 97 (10)
Osteoclast Lineage Development
Toshiyuki Yamane
Takahiro Kunisada
Shin-Ichi Hayashi
Differentation of Embryonic Stem Cells as a 107(10)
Model to Study Gene Function During the
Development of Adipose Cells
Christian Dani
Embryonic Stem Cell Differentiation and the 117(10)
Vascular Lineage
Victoria L. Bautch
Embryonic Stem Cells as a Model to Study 127(30)
Cardiac, Skeletal Muscle, and Vascular Smooth
Muscle Cell Differentiation
Anna M. Wobus
Kaomei Guan
Huang-Tia Yang
Kenneth R. Boheler
Cardiomyocyte Enrichment in Differentiating 157(12)
ES Cell Cultures: Strategies and Applications
Kishore B. S. Pasumarthi
Loren J. Field
Embryonic Stem Cells as a Model for the 169(20)
Physiological Analysis of the Cardiovascular
Jurgen Hescheler
Maria Wartenberg
Bernd K. Fleischmann
Kathrin Banach
Helmut Acker
Heinrich Sauer
Isolation of Lineage-Restricted Neural 189(16)
Precursors From Cultured ES Cells
Tahmina Mujtaba
Mahendra S. Rao
Lineage Selection for Generation and 205(12)
Amplification of Neural Precursor Cells
Meng Li
Selective Neural Induction from ES Cells by 217(12)
Stromal Cell-Derived Inducing Activity and
its Potential Therapeutic Application in
Parkinson's Disease
Hiroshi Kawasaki
Kenji Mizuseki
Yoshiki Sasai
Epidermal Lineage 229(26)
Tammy-Claire Troy
Kursad Turksen
ES Cell Differentiation Into the Hair 255(6)
Follicle Lineage In Vitro
Tammy-Claire Troy
Kursed Turksen
Embryonic Stem Cells as a Model for Studying 261(8)
Melanocyte Development
Toshiyuki Yamane
Shin-Ichi Hayashi
Takahiro Kunisada
Using Progenitor Cells and Gene Chips to 269(16)
Define Genetic Pathways
S. Steven Potter
M. Todd Valerius
Eric W. Brunskill
ES Cell-Mediated Conditional Transgenesis 285(24)
Marina Gertsenstein
Corrinne Lobe
Andras Nagy
Switching on Lineage Tracers Using 309(26)
Site-Specific Recombination
Susan M. Dymecki
Carolyn I. Rodriguez
Rajeshwar B. Awatramani
From ES Cells to Mice: The Gene Trap Approach 335(12)
Francesco Cecconi
Peter Gruss
Functional Genomics by Gene-Trapping in 347(34)
Embryonic Stem Cells
Thomas Floss
Wolfgang Wurst
Phage-Displayed Antibodies to Detect Cell 381(12)
Jun Lu
Steven R. Sloan
Gene Transfer Using Targeted Filamentous 393(10)
David Larocca
Kristen Jensen-Pergakes
Michael A Burg
Andrew Baird
Single-Cell PCR Methods for Studying Stem 403(14)
Cells and Progentiors
Jane E. Aubin
Fina Liu
G. Antonio Candeliere
Nonradioactive Labeling and Detection of 417(8)
mRNAs Hybridized onto Nucleic Acid cDNA Arrays
Thorsten Hoevel
Manfred Kubbies
Expression Profiling Using Quantitative 425(8)
Hybridization on Macroarrays
Genevieve Pietu
Charles Decraene
Isolation of Antigen-Specific Intracellular 433(14)
Antibody Fragments as Single Chain Fv for Use
in Mammalian Cells
Eric Tse
Grace Chung
Terence H. Rabbitts
Detection and Visualization of Protein 447(14)
Interactions with Protein Fragment
Complementation Assays
Ingrid Remy
Andre Galarneau
Stephen W. Michnick
Direct Selection of cDNAs by Phage Display 461(10)
Reto Crameri
Gernot Achatz
Michael Wichel
Claudio Rhyner
Screening for Protein-Protein Interactions in 471(16)
the Yeast Two-Hybrid System
R. Daniel Gietz
Robin A. Woods
Index 487