Oxidative Stress Biomarkers and Antioxidant Protocols (Methods in Molecular Biology)


Oxidative Stress Biomarkers and Antioxidant Protocols (Methods in Molecular Biology)

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  • 製本 Hardcover:ハードカバー版/ページ数 336 p.
  • 言語 ENG
  • 商品コード 9780896038509
  • DDC分類 616.07

Full Description

The first protocols book, Free Radical and Antioxidant Protocols (1) was published in late 1998. Sections were divided into three parts, covering selected biochemical techniques for measuring oxidative stress, antioxidant (AOX) activity, and combined applications. In choosing the 40 methods to be included in that book, I realized there were considerably more of equal value than that which we could have presented in a single volume. To produce a comprehensive resource, this book and a third are being compiled to expand coverage of the field. A summary of papers (2) published on this important subject emphasizes the continuing rapid growth in oxidative stress investigations relating to our understanding of biochemical reactions, their relevance to pathophysiological mechanisms, how disease may arise, and how therapeutic intervention may be achieved(3). Although there is some overlap between the categories, the ana- sis shown below illustrates where current studies are concentrated and are almost evenly distributed between free radicals and AOX. Over the last 4 yr, there has been a 55% increase in the number of papers published in the area.


Part I. Techniques for Free Radical Derived Biomarkers.

Human Xanthine Oxidoreductase Determination by a Competitive ELISA
Maria Giulia Battelli and Silvia Musiani

Simultaneous Determination of Polyunsaturated Fatty Acids and Corresponding Monohydroperoxy and Monohydroxy Peroxidation Products by HPLC
Richard W. Browne and Donald Armstrong

Determination of Products of Lipid Oxidation by Infrared Spectroscopy
Douglas Borchman and Santosh Sinha

Detection of Docosahexaenoic Acid Hydroperoxides in Retina by Gas Chromatography/Mass Spectrometry
Guey-Shuang Wu and Narsing A. Rao

Detection of Lipid Hydroperoxide-Derived Protein Modification with Polyclonal Antibodies
Yoji Kato and Toshihiko Osawa

Techniques for Determining the Metabolic Pathways of Eicosanoids and for Evaluating the Rate-Controlling Enzymes
Ninder Panesar, Yashwant G. Deshpande, and Donald L. Kaminski

Mass Spectrometric Quantification of F2-Isoprostanes as Indicators of Oxidative Stress
Jason D. Morrow and L. Jackson Roberts, II

Formation of Apolipoprotein AI-AII Heterodimers by Oxidation of High-Density Lipoprotein
Audric S. Moses and Gordon A. Francis

Detection of Certain Peroxynitrite-Induced DNA Modifications
Hiroshi Ohshima, László Virág, Jose Souza, Vladimir Yermilov, Brigitte Pignatelli, Mitsuharu Masuda, and Csaba Szabó

Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS
José A. Castro and Gerardo D. Castro

Analysis of Aliphatic Amino Acid Alcohols in Oxidized Proteins
Bénédicte Morin, Shanlin Fu, Hongjie Wang, Michael J. Davies, and Roger T. Dean

Rapid Determination of Glutamate Using HPLC Technology
Aqeela Afzal, Mohammed Afzal, Andrew Jones, and Donald Armstrong

A Rapid Method for the Quantification of GSH and GSSG in Biological Samples
Mohammed Afzal, AqeelaAfzal, Andrew Jones, and Donald Armstrong

Protein Carbonyl Measurement by ELISA
I. Hendrikje Buss and Christine C. Winterbourn

Ne-(carboxymethyl)lysine (CML) as a Biomarker of Oxidative Stress in Long-Lived Tissue Proteins
J. Nikki Shaw, John W. Baynes, and Suzanne R. Thorpe

Measurement of S-Glutathionated Hemoglobin in Human Erythrocytes by Isoelectric Focusing Electrophoresis
Haw-Wen Chen and Chong-Kuei Lii

Oxidation of Cellular DNA Measured with the Comet Assay
Andrew R. Collins and Mária Duþs inská

Measurement of DNA Double-Strand Breaks with Giant DNA and High Molecular-Weight DNA Fragments by Pulsed-Field Gel Electrophoresis
Yoshihiro Higuchi

Evaluation of Antibodies Against Oxygen Free Radical-Modified DNA by ELISA
Rashid Ali and Khurshid Alam

Part II. Techniques for Antioxidant Biomarkers

Simultaneous Analysis of Multiple Redox-Active Metabolites from Biological Matrices
Bruce S. Kristal, Karen Vigneau-Callahan, and Wayne R. Matson

Determination of Uric Acid in Urine by Fast-Scan Voltammetry (FSV) Using a Highly Activated Carbon Fiber Electrode
Roberto Bravo, Dawn M. Stickle, and Anna Brajter-Toth

Measurement of a-Tocopherol Turnover in Plasma and in Lipoproteins Using Stable Isotopes and Gas Chromatography/Mass Spectrometry
Elizabeth J. Parks

Analysis of Tocotrienols in Different Sample Matrixes by HPLC
Kalyana Sundram and Rosnah Md. Nor

Measurement of b-Carotene15,15'-Dioxygenase Activity by Reverse-Phase HPLC
Alexandrine During, Akihiko Nagao, and James Cecil Smith, Jr.

Ubiquinol/Ubiquinone Ratio as a Marker of Oxidative Stress
Yorihiro Yamamoto and Satoshi Yamashita

Catechol- and Pyrogallol-Type Flavonoids: Analysis of Tea Catechins in Plasma
Keizo Umegaki, Mituaki Sano, and Isoa Tomita

Pyruvate Dehydrogenase Complex as a