The study of the interactions of proteins, whether they be enzymes, regulatory proteins or structural proteins, with nucleic acids is one of the most exciting areas of modern molecular biology. In this 1993 text, Professor Steitz reviews the wide-ranging research in structural studies of DNA-binding proteins and their complexes with DNA. The author clearly and concisely describes the uses of techniques in molecular genetics, DNA synthesis, protein crystallography and nuclear magnetic response. The book, by a now Nobel Prize-winning author, will continue to be enjoyed by anyone with an interest in this exciting area of research.
1. Introduction and overview; 2. Principles of sequence-specific nucleic-acid recognition; 3. DNA-binding structure motifs; 4. Similarities and differences in RNA and DNA recognition; 5. Sequence-specific DNA-binding proteins; 6. Sequence-independent DNA-binding proteins; 7. Sequence-specific RNA-binding proteins; 8. Conclusions and prospects; 9. Acknowledgements; 10. References; Index.