Combines histochemistry with molecular biology and enables the rapid analysis of the distribution of RNA, or DNA, in the tissues.
Advances in our understanding of biological mechanisms have frequently been associated with the development of techniques. In situ hybridisation is a classic case of just such an advance. The technique effectively combines histochemistry with molecular biology and enables the rapid analysis of the distribution of RNA, or DNA, in the tissues. The information gained from this has caused something of a revolution in our understanding of developmental biology, since a fundamental aspect of development is the spatial and temporal expression of genes. In addition the technique has found application in the field of medicine, providing insights into the functioning of healthy tissues and the diagnosis and study of diseases. This book brings together contributions from leaders in the application of in situ hybridisation and guides the would-be exponent through the various options and variations of the technique.
Table of Contents
1. Non radio isotopic labels for in situ
hybridisation histochemistry: a histochemist's
view G. Coulton
2. Use of haptenised nucleic acid probes in
fluorescent in situ hybridisation A. K. Raap
3. The use of complementary RNA probes for the
identification and localisation of peptide
messenger RNA in the diffuse neuroendocrine
system A. Giaid
4. Contributions of the spatial analysis of
gene expression to the study of sea urchin
development R. C. Aangerer
5. Advantages and limitations of in situ
hybridisation as exemplified by the molecular
genetic analysis of Drosophila development P.
6. The use of in situ hybridisation to study
the localisation of maternal mRNAs during
Xenopus oogenesis H. Perry-O'Keefe
7. In situ hybridisation in the analysis of
genes with potential roles in mouse
embryogenesis D. G. Wilkinson
8. Evolution of algal plasmids from eukaryotic
endosymbionts G. I. McFadden
9. Localisation of expression of male flower
specific genes from maize by in situ
hybridisation S. Y. Wright and A. J. Greenland
10. Tissue preparation techniques for in situ
hybridisation studies of storage-protein gene
expression during pea seed development N. Harris
11. Investigation of gene expression during
plant gametogenesis by in situ hybridisation K.
12. Sexing the human conceptus by in situ
hybridisation J. D. West
13. Nonisotopic in situ hybridisation in human
pathology C. S. Herrington
14. The demonstration of viral DNA in human
tissues by in situ hybridisation M. Wells.